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Anti-XPA antibody [5F12] (ab65963)

Price and availability

268 032 ₸

Availability

Order now and get it on Tuesday February 23, 2021

Anti-XPA antibody [5F12] (ab65963)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [5F12] to XPA
  • Suitable for: WB, ICC/IF
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG2b

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Overview

  • Product name

    Anti-XPA antibody [5F12]
    See all XPA primary antibodies
  • Description

    Mouse monoclonal [5F12] to XPA
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant full length XPA protein (Human)

  • Epitope

    Amino acids 30-47.
  • Positive control

    • WB: HeLa and 293T cell lysates.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 6
    Constituents: PBS, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Ion Exchange Chromatography
  • Clonality

    Monoclonal
  • Clone number

    5F12
  • Isotype

    IgG2b
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Nucl. Excision Repair

Images

  • Western blot - Anti-XPA antibody [5F12] (ab65963)
    Western blot - Anti-XPA antibody [5F12] (ab65963)
    All lanes : Anti-XPA antibody [5F12] (ab65963) at 1/500 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : XPA knockout HeLa cell lysate
    Lane 3 : 293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 31 kDa
    Observed band size: 38 kDa
    why is the actual band size different from the predicted?



    Lanes 1-3: Merged signal (red and green). Green - ab65963 observed at 38 kDa. Red - loading control, ab52866 observed at 50 kDa.

    ab65963 Anti-XPA antibody [5F12] was shown to specifically react with XPA in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264663 (knockout cell lysate ab258764) was used. Wild-type and XPA knockout samples were subjected to SDS-PAGE. ab65963 and Anti-alpha Tubulin antibody [EP1332Y] - Loading Control (ab52866) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-XPA antibody [5F12] (ab65963)
    Immunocytochemistry/ Immunofluorescence - Anti-XPA antibody [5F12] (ab65963)

    Immunofluorescent staining of human fibroblast cells (GM0637) using ab65963 at a dilution of 1/100. The cells were non-irradiated (left) or irradiated with UV at 20 J/m2 (right), fixed after 30 minutes with paraformaldehyde and counter-stained with Hoescht. The top panels show the antibody staining, the middle panels show the Hoescht staining and the bottom panels are a merge of the two images. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-mouse IgG, used at a dilution of 1/5000.

  • Western blot - Anti-XPA antibody [5F12] (ab65963)
    Western blot - Anti-XPA antibody [5F12] (ab65963)
    Anti-XPA antibody [5F12] (ab65963) at 0.1 µg/ml + Crude extract of HeLa cells at 200 µg

    Predicted band size: 31 kDa
    Observed band size: 38 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-XPA antibody [5F12] (ab65963)
    Western blot - Anti-XPA antibody [5F12] (ab65963)
    All lanes : Anti-XPA antibody [5F12] (ab65963) at 1/2000 dilution

    Lane 1 : HeLa cell extract (XPA wild type)
    Lane 2 : XP12ROSV cell extract (XPA deficient)

    Predicted band size: 31 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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