Anti-WSTF antibody [EPR1703] (ab109439)
![Anti-WSTF antibody [EPR1703] (ab109439)](https://www.abcam.com/ps/products/109/ab109439/Images/ab109439-448878-anti-wstf-antibody-epr1703-western-blot-hela-lysates.jpg "Anti-WSTF antibody [EPR1703] (ab109439)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1703] to WSTF
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-WSTF antibody [EPR1703]
See all WSTF primary antibodies -
Description
Rabbit monoclonal [EPR1703] to WSTF -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HAP1, 293T, HeLa, HT-1080, PC-12, and SH-SY5Y cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.23 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR1703 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-WSTF antibody [EPR1703] (ab109439)All lanes : Anti-WSTF antibody [EPR1703] (ab109439) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : WSTF knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 171 kDa
Observed band size: 171 kDaLanes 1- 2: Merged signal (red and green). Green - ab109439 observed at 171 kDa. Red - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) observed at 50 kDa.
ab109439 was shown to react with WSTF in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab264907 (knockout cell lysate ab257370) was used. Wild-type HeLa and WSTF knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109439 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-WSTF antibody [EPR1703] (ab109439)](https://www.abcam.com/ps/products/109/ab109439/Images/ab109439-274332-anti-wstf-antibody-epr1703-western-blot.jpg)
Western blot - Anti-WSTF antibody [EPR1703] (ab109439)All lanes : Anti-WSTF antibody [EPR1703] (ab109439) at 1/1000 dilution
Lane 1 : Wild type HAP1 whole cell lysate
Lane 2 : BAZ1B knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 171 kDaLanes 1 - 3: Merged signal (red and green). Green - ab109439 observed at 171 kDa. Red - loading control, ab18058, observed at 130 kDa.
Ab109439 was shown to recognize BAZ1B in wild-type cells along with additional cross-reactive bands as signal was lost in BAZ1B knockout samples. Wild-type and BAZ1B knockout samples were subjected to SDS-PAGE. Ab109439 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
![Western blot - Anti-WSTF antibody [EPR1703] (ab109439)](https://www.abcam.com/ps/products/109/ab109439/Images/ab109439-122285-anti-wstf-antibody-epr1703-western-blot.jpg)
Western blot - Anti-WSTF antibody [EPR1703] (ab109439)All lanes : Anti-WSTF antibody [EPR1703] (ab109439) at 1/1000 dilution
Lane 1 : 293T cell lysates
Lane 2 : HeLa cell lysates
Lane 3 : HT-1080 cell lysates
Lane 4 : PC-12 cell lysates
Lane 5 : SH-SY5Y cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 171 kDa
Observed band size: 185 kDa why is the actual band size different from the predicted?
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![OI-RD Scanning - Anti-WSTF antibody [EPR1703] (ab109439)](https://www.abcam.com/ps/products/109/ab109439/Images/ab109439-122284-anti-wstf-antibody-epr1703.jpg)
OI-RD Scanning - Anti-WSTF antibody [EPR1703] (ab109439) -
![Anti-WSTF antibody [EPR1703] (ab109439)](https://www.abcam.com/ps/products/109/ab109439/Images/ab109439-5-benefits-of-recombinant-antibodies.png)
Anti-WSTF antibody [EPR1703] (ab109439)
