Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-WSTF antibody [EP1704Y] - BSA and Azide free (ab235388)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1704Y] to WSTF - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-WSTF antibody [EP1704Y] - BSA and Azide free
    See all WSTF primary antibodies

  • Description

    Rabbit monoclonal [EP1704Y] to WSTF - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Wild-type HAP1, HeLa (ab150035), and PC12 cell lysates. Mouse and Rat testis. B16-F0, MCF7, HeLa whole cell lysates. Flow cyt: HeLa cells; IHC: rat cardiac muscle tissue, mouse cardiac muscle tissue, human breast carcinoma; ICC: HeLa cells.

  • General notes

    ab235388 is the carrier-free version of ab51256.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-WSTF antibody [EP1704Y] - BSA and Azide free (ab235388)
    Western blot - Anti-WSTF antibody [EP1704Y] - BSA and Azide free (ab235388)
    All lanes : Anti-WSTF antibody [EP1704Y] (ab51256) at 1/15000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : BAZ1B knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 171 kDa
    Observed band size: 175 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab51256).

      Lanes 1- 2: Merged signal (red and green). Green - ab51256 observed at 175 kDa. Red - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) observed at 50 kDa.

     ab51256 was shown to react with WSTF in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab264907 (knockout cell lysate ab257370) was used. Wild-type HeLa and BAZ1B knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab51256 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) overnight at 4°C at a 1 in 15000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-WSTF antibody [EP1704Y] - BSA and Azide free (ab235388)
    Immunocytochemistry/ Immunofluorescence - Anti-WSTF antibody [EP1704Y] - BSA and Azide free (ab235388)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling WSTF with ab51256 at a dilution of 1/1000. Cells were fized with 4% paraformaldehyde and permeabilized with 0.1% tritonX-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody at a dilution of 1/1000.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51256).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WSTF antibody [EP1704Y] - BSA and Azide free (ab235388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WSTF antibody [EP1704Y] - BSA and Azide free (ab235388)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue labelling WSTF with ab51256 at a dilution of 1/500. Heat mediated antigen retrieval was performed using citrate buffer, pH 6.0. A ready to use HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody. Counter stained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51256).

  • Western blot - Anti-WSTF antibody [EP1704Y] - BSA and Azide free (ab235388)
    Western blot - Anti-WSTF antibody [EP1704Y] - BSA and Azide free (ab235388)
    All lanes : Anti-WSTF antibody [EP1704Y] (ab51256) at 1/15000 dilution

    Lane 1 : Wild-type HAP1 cell lysate
    Lane 2 : WSTF knockout HAP1 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : PC12 cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 171 kDa



    This WB data was generated using the same anti-WSTF antibody clone, EP1704Y, in a different buffer formulation (cat# ab51256).


    Lanes 1 - 4: Merged signal (red and green). Green - ab51256 observed at 175 kDa. Red - loading control, ab18058, observed at 124 kDa.

    ab51265 was shown to recognize WSTF in wilt-type cells along with additional cross-reactive bands as signal was lost in WSTF knockout samples. Wild-type and WSTF knockout samples were subjected to SDS-PAGE. ab51256 and ab18058 (loading control to vinculin) were diluted 1/15 000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WSTF antibody [EP1704Y] - BSA and Azide free (ab235388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WSTF antibody [EP1704Y] - BSA and Azide free (ab235388)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cardiac musle tissue labelling WSTF with ab51256 at a dilution of 1/500. Heat mediated antigen retrieval was performed using citrate buffer, pH 6.0. A ready to use HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody. Counter stained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51256).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WSTF antibody [EP1704Y] - BSA and Azide free (ab235388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WSTF antibody [EP1704Y] - BSA and Azide free (ab235388)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cardiac muscle tissue labelling WSTF with ab51256 at a dilution of 1/500. Heat mediated antigen retrieval was performed using citrate buffer, pH 6.0. A ready to use HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody. Counter stained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51256).

  • Anti-WSTF antibody [EP1704Y] - BSA and Azide free (ab235388)
    Anti-WSTF antibody [EP1704Y] - BSA and Azide free (ab235388)

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