Anti-Wnt3 antibody (ab50341)
Key features and details
- Goat polyclonal to Wnt3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Wnt3 antibody
See all Wnt3 primary antibodies -
Description
Goat polyclonal to Wnt3 -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide:
CGRGHNTRTEKRKEK
, corresponding to internal sequence amino acids 315-329 of Human Wnt3
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3 antibody (ab50341)
ab50341 (2µg/ml) staining of paraffin embedded Human Breast shows cytoplasm staining in some luminal epithelial cells following steamed antigen retrieval with citrate buffer pH 6 and HRP-staining.
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Western blot - Anti-Wnt3 antibody (ab50341)Anti-Wnt3 antibody (ab50341) at 1 µg/ml + Human Brain lysate (35µg protein in RIPA buffer).
Predicted band size: 40 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Additional bands at: 17 kDa. We are unsure as to the identity of these extra bands.
Primary incubation was 1 hour. Detected by chemiluminescence. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3 antibody (ab50341)ab50341 at 3.8µg/ml staining Wnt3 in Human breast tissue section by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The tissue underwent antigen retrieval by steam in citrate buffer pH 6.0. An AP staining procedure was used.
