Anti-PHOX2A antibody (ab130121)
Key features and details
- Rabbit polyclonal to PHOX2A
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PHOX2A antibody
See all PHOX2A primary antibodies -
Description
Rabbit polyclonal to PHOX2A -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Mouse PHOX2A aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab151604) -
Positive control
- This antibody gave a positive signal in the following whole cell lysates: SHSY5Y; SKNBE; PC12.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab130121 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRabbitHorseCowPigMacaque monkeyOrangutanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 29 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 29 kDa).Target
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Function
May be involved in regulating the specificity of expression of the catecholamine biosynthetic genes. Acts as a transcription activator/factor. Could maintain the noradrenergic phenotype. -
Involvement in disease
Defects in PHOX2A are the cause of congenital fibrosis of extraocular muscles type 2 (CFEOM2) [MIM:602078]. CFEOM encompasses several different inherited strabismus syndromes characterized by congenital restrictive ophthalmoplegia affecting extraocular muscles innervated by the oculomotor and/or trochlear nerves. CFEOM is characterized clinically by anchoring of the eyes in downward gaze, ptosis, and backward tilt of the head. CFEOM2 may result from the aberrant development of the oculomotor (nIII), trochlear (nIV) and abducens (nVI) cranial nerve nuclei. -
Sequence similarities
Belongs to the paired homeobox family.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 507670 Cow
- Entrez Gene: 401 Human
- Entrez Gene: 11859 Mouse
- Entrez Gene: 116648 Rat
- Omim: 602753 Human
- SwissProt: O14813 Human
- SwissProt: Q62066 Mouse
- SwissProt: Q62782 Rat
see all -
Alternative names
- Aristaless homeobox (Drosophila) fibrosis of extraocular muscles congenital 2 autosomal recessive antibody
- Aristaless homeobox gene homolog (Drosophila) antibody
- Aristaless homeobox homolog antibody
see all
Images
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Western blot - Anti-PHOX2A antibody (ab130121)All lanes : Anti-PHOX2A antibody (ab130121) at 1 µg/ml
Lane 1 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 2 : SK N BE (Human neuroblastoma) Whole Cell Lysate
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate - Negative Control
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 34,36 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa, 200 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
The predicted molecular weight of PHOX2A is 29 kDa (SwissProt), however we expect to observe a banding pattern around 36 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. Please note that HEK293 (lane 4) was used as a negative control for PHOX2A. This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab130121 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
References (0)
ab130121 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-PHOX2A antibody (ab130121)All lanes : Anti-PHOX2A antibody (ab130121) at 1 µg/ml
Lane 1 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 2 : SK N BE (Human neuroblastoma) Whole Cell Lysate
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate - Negative Control
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 34,36 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa, 200 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
The predicted molecular weight of PHOX2A is 29 kDa (SwissProt), however we expect to observe a banding pattern around 36 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. Please note that HEK293 (lane 4) was used as a negative control for PHOX2A. This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab130121 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
