Anti-WISP3 antibody (ab224720)
Key features and details
- Rabbit polyclonal to WISP3
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-WISP3 antibody
See all WISP3 primary antibodies -
Description
Rabbit polyclonal to WISP3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human WISP3 aa 200 to the C-terminus (C terminal).
Database link: O95389-2 -
Positive control
- WB: HepG2 whole cell lysate (ab7900); Mouse heart, brain and kidney lysates; Rat heart and brain lysates. IHC-P: Human liver cancer and kidney tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-WISP3 antibody (ab224720)All lanes : Anti-WISP3 antibody (ab224720) at 1/500 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 2 : Rat brain lysate
Lane 3 : Rat heart lysate
Lane 4 : Mouse heart lysate
Lane 5 : Mouse brain lysate
Lane 6 : Mouse kidney lysate
Secondary
All lanes : Goat Anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 40, 42 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WISP3 antibody (ab224720)Paraffin-embedded human kidney tissue stained for WISP3 using ab224720 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WISP3 antibody (ab224720)Paraffin-embedded human liver cancer tissue stained for WISP3 using ab224720 at 1/100 dilution in immunohistochemical analysis.
