KG-1 nuclear extract lysate (ab14854)
Overview
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Product name
KG-1 nuclear extract lysate -
General notes
Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.90
Constituents: 0.75% Potassium chloride, 0.009% PMSF, 0.01% Magnesium chloride, 0.008% DTT, 0.48% HEPES, 20% Glycerol -
Concentration information loading... -
Research areas
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Background
Myeloid dendritic cells (DC) are representatives of a rare and phenotypically diverse population of professional antigen presenting cells possessing high functional heterogeneity and flexibility. KG-1 cells are used as an erythroleukemia model cell line, which shares morphological and physiological similarities with immature and mature myeloid DC. These cells posses numerous tumor markers and other antigens (DR, Ia-like antigens) and are used in studies of tumorigenicity, differentiation. They are CD3 negative, CD4 negative, CD13 positive , CD14 negative, CD15 positive, CD19 negative, CD33 positive, CD34 positive, HLA-DR positive.