Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-West Nile Virus NS1 antibody [IC12.E7.G9/01] (ab253236)

Key features and details

  • Mouse monoclonal [IC12.E7.G9/01] to West Nile Virus NS1
  • Suitable for: ELISA
  • Reacts with: West Nile virus
  • Isotype: IgG1

Overview

  • Product name

    Anti-West Nile Virus NS1 antibody [IC12.E7.G9/01]

  • Description

    Mouse monoclonal [IC12.E7.G9/01] to West Nile Virus NS1

  • Host species

    Mouse

  • Specificity

    This antibody is specific for the NS1 protein of West Nile virus, and does not cross-react with NS1 from other flaviviruses, including Dengue virus serotypes 1- 4, Zika virus, Yellow Fever virus and Japanese Encephalitis virus. No cross-reactivity is seen with Chikungunya virus E1, E2 or C proteins.

  • Tested applications

    Suitable for: ELISAmore details

  • Species reactivity

    Reacts with: West Nile virus

  • Immunogen

    Recombinant full length protein corresponding to West Nile virus West Nile Virus NS1.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    Purified from tissue culture supernatant.

  • Clonality

    Monoclonal

  • Clone number

    IC12.E7.G9/01

  • Isotype

    IgG1

  • Research areas

Images

  • ELISA - Anti-West Nile Virus NS1 antibody [IC12.E7.G9/01] (ab253236)
    ELISA - Anti-West Nile Virus NS1 antibody [IC12.E7.G9/01] (ab253236)

    ELISA plates coated on bench overnight in 0.1M carbonate pH 9.6, 100 µl/well, washed once in wash buffer 300 µl/well (TBS + 0.1% tween 20) and blocked 2 hrs in 1% BSA in D-PBS 300 µl/well. Antibodies diluted to working strength in diluent (DPBS + 1% BSA + 0.05% Tween 20 + 0.2% Proclin 950). Added at 100 µl/well and incubated 2 hrs shaken at ambient temperature. Washed 3 x 300 µl/well. Goat anti Mouse IgG-HRP diluted 1/2500 in diluent, added at 100 µl/well and incubated with shaking 1 hr at ambient. Plate washed 6X300 µl/well. TMB added at 100 µl/well. Reaction for screening assay stopped by addition of 1M HCl 100 µl/well.

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