Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10709(2)] to WDR77 - BSA and Azide free
  • Suitable for: ICC, IP, WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free
    See all WDR77 primary antibodies

  • Description

    Rabbit monoclonal [EPR10709(2)] to WDR77 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Rat
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251023 is the carrier-free version of ab190361. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251023 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Images

  • Western blot - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)
    Western blot - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)
    All lanes : Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361) at 1/10000 dilution

    Lane 1 : LnCaP cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 37 kDa
    Observed band size: 42 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab190361, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)
    Western blot - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)
    Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361) at 1/1000 dilution + Saos-2 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 37 kDa
    Observed band size: 42 kDa why is the actual band size different from the predicted?



    This data was developed using ab190361, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)
    Western blot - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)
    All lanes : Anti-WDR77 antibody [EPR10709(2)] - C-terminal (ab190361) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : Raw 264.7 cell lysate
    Lane 3 : PC-12 cell lysate
    Lane 4 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 37 kDa
    Observed band size: 42 kDa why is the actual band size different from the predicted?



    This data was developed using ab190361, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)
    This data was developed using ab190361, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human adenocarcinoma of the colon labeling WDR77 with ab190361 at 1/50 and HRP polymer for Rabbit IgG. Counterstained with Hematoxylin. Negative control also shown. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)
    Immunocytochemistry - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)
    This data was developed using ab190361, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of A673 cells fixed in 4% paraformaldehyde labeling WDR77 with ab190361 at 1/50 dilution and Goat anti rabbit IgG (Alexa Fluor® 555) 1/200. Counterstained with DAPI. Negative controls also shown.
  • Flow Cytometry - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)
    Flow Cytometry - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)
    This data was developed using ab190361, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling WDR77 at 1/70 dilution and Goat anti rabbit IgG (FITC) at 1/150. Rabbit monoclonal IgG used as isotype control.
  • Immunoprecipitation - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)
    Immunoprecipitation - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)
    This data was developed using ab190361, the same antibody clone in a different buffer formulation.Immunoprecipitation of HeLa cells labeling WDR77 with ab190361 at 1/50 and Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/5000 (+) or PBS (-)
  • Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)
    Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)

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