Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IMPA2 antibody [EPR23668-64] - BSA and Azide free (ab280576)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23668-64] to IMPA2 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-IMPA2 antibody [EPR23668-64] - BSA and Azide free
    See all IMPA2 primary antibodies

  • Description

    Rabbit monoclonal [EPR23668-64] to IMPA2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt (Intra),ICC or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MDA-MB-468, HeLa, RAW264.7, PC-12 and MCF7 transfected with IMPA2 expression vector lysates. IP: MDA-MB-468 cells.

  • General notes

    ab280576 is the carrier-free version of ab256410. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab280576 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-IMPA2 antibody [EPR23668-64] - BSA and Azide free (ab280576)
    Western blot - Anti-IMPA2 antibody [EPR23668-64] - BSA and Azide free (ab280576)
    All lanes : Anti-IMPA2 antibody [EPR23668-64] (ab256410) at 1/1000 dilution

    Lane 1 : MDA-MB-468 (human breast adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
    Lane 4 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 31 kDa



    This data was developed using ab256410, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The observed MW is consistent with what has been described in the literature (PMID: 23205734).

    Exposure time: Lanes 1-2: 3 minutes; Lanes 3-4: 81 seconds.

     

  • Immunoprecipitation - Anti-IMPA2 antibody [EPR23668-64] - BSA and Azide free (ab280576)
    Immunoprecipitation - Anti-IMPA2 antibody [EPR23668-64] - BSA and Azide free (ab280576)

    This data was developed using ab256410, the same antibody clone in a different buffer formulation.

    IMPA2 was immunoprecipitated from 0.35 mg MDA-MB-468 (human breast adenocarcinoma epithelial cell), whole cell lysate 10 µg with ab256410 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256410 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: MDA-MB-468 whole cell lysate 10 µg

    Lane 2: ab256410 IP in MDA-MB-468 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab256410 in MDA-MB-468 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 24 seconds

  • Western blot - Anti-IMPA2 antibody [EPR23668-64] - BSA and Azide free (ab280576)
    Western blot - Anti-IMPA2 antibody [EPR23668-64] - BSA and Azide free (ab280576)
    All lanes : Anti-IMPA2 antibody [EPR23668-64] (ab256410) at 1/1000 dilution

    Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell) transfected with an empty vector (vector control), whole cell lysate
    Lane 2 : MCF7 (human breast adenocarcinoma epithelial cell) transfected with IMPA2 expression vector, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 31 kDa



    This data was developed using ab256410, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Lysates were kindly provided by an anonymous collaborator.

    Exposure time: 3.25 seconds.

  • Anti-IMPA2 antibody [EPR23668-64] - BSA and Azide free (ab280576)
    Anti-IMPA2 antibody [EPR23668-64] - BSA and Azide free (ab280576)

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