Anti-WDR45 antibody (ab240905)
Key features and details
- Rabbit polyclonal to WDR45
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-WDR45 antibody
See all WDR45 primary antibodies -
Description
Rabbit polyclonal to WDR45 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human WDR45 aa 312-322 (C terminal).
Database link: Q9Y484 -
Positive control
- ICC/IF: HeLa cells. WB: HEK-293Trap and HeLa cell lysats.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-WDR45 antibody (ab240905)
4% Formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for WDR45 (Green) using ab240905 at 1/100 dilution for 60 minutes at RT. Anti-Rabbit ATTO 488 at 1:200 dilution was used as the secondary antibody for 60 minutes at RT. Phalloidin Texas Red F-Actin stain was used as the counterstain at 1/1000 dilution for 60 minutes at RT. The nuclear counterstain was DAPI (Blue) for 5 minutes at RT. Localization: Cytoplasm . Magnification: 60X.
A: DAPI, B: Actin, C: WDR45, D: Composite.
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Western blot - Anti-WDR45 antibody (ab240905)All lanes : Anti-WDR45 antibody (ab240905) at 1/1000 dilution (1 hour at RT)
Lane 1 : HeLa whole cell lysate
Lane 2 : HEK-293Trap whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT
Developed using the ECL technique.
Exposure time: 6 minutesBlock: 5% Skim Milk in 1X TBST.