Anti-WDR12 antibody [1B8] - BSA and Azide free (ab255883)
![Anti-WDR12 antibody [1B8] - BSA and Azide free (ab255883)](https://www.abcam.com/ps/products/255/ab255883/Images/ab255883-390672-anti-wdr12-antibody-1b8-western-blot-hela.jpg "Anti-WDR12 antibody [1B8] - BSA and Azide free (ab255883)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [1B8] to WDR12 - BSA and Azide free
- Suitable for: WB, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-WDR12 antibody [1B8] - BSA and Azide free
See all WDR12 primary antibodies -
Description
Rat monoclonal [1B8] to WDR12 - BSA and Azide free -
Host species
Rat -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human WDR12. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9GZL7 -
Positive control
- WB: HeLa, HEK-293, Jurkat and HepG2 whole cell lysate. ICC/IF: HEK-293 and HeLa cell.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
ab255883 is the carrier-free version of ab252878. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
1B8 -
Isotype
IgG1 -
Light chain type
lambda
Images
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Western blot - Anti-WDR12 antibody [1B8] - BSA and Azide free (ab255883)All lanes : Anti-WDR12 antibody [1B8] (ab252878) at 1.235 µg/ml
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : HEK-293 (human embryonic kidney epithelial cell), whole cell lysate
Lane 3 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 4 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution
Predicted band size: 48 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: Lanes 1/3: 3 seconds; Lane 2 : 15 seconds; Lane 4: 1 second.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252878).
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![Immunocytochemistry/ Immunofluorescence - Anti-WDR12 antibody [1B8] - BSA and Azide free (ab255883)](https://www.abcam.com/ps/products/255/ab255883/Images/ab255883-2-anti-wdr12-antibody-1b8-immunocytochemistry-hela.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-WDR12 antibody [1B8] - BSA and Azide free (ab255883)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling WDR12 with ab252878 at 12.35 µg/mL, followed by ab150157 Goat Anti-rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nucleolar staining in HeLa cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150157 Goat Anti-rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252878).
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![Immunocytochemistry/ Immunofluorescence - Anti-WDR12 antibody [1B8] - BSA and Azide free (ab255883)](https://www.abcam.com/ps/products/255/ab255883/Images/ab255883-1-anti-wdr12-antibody-1b8-immunocytochemistry-hek293.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-WDR12 antibody [1B8] - BSA and Azide free (ab255883)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293 cells labelling WDR12 with ab252878 at 12.35 µg/mL, followed by ab150157 Goat Anti-rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nucleolar staining in HEK-293 cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150157 Goat Anti-rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252878).
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![Anti-WDR12 antibody [1B8] - BSA and Azide free (ab255883)](https://www.abcam.com/ps/products/255/ab255883/Images/ab255883-4-benefits-of-recombinant-antibodies.png)
Anti-WDR12 antibody [1B8] - BSA and Azide free (ab255883)
