Anti-WDFY3 antibody - N-terminal (ab240917)
Key features and details
- Rabbit polyclonal to WDFY3 - N-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-WDFY3 antibody - N-terminal
See all WDFY3 primary antibodies -
Description
Rabbit polyclonal to WDFY3 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide corresponding to Human WDFY3 aa 500-600 (N terminal). NP_055806.2
Database link: Q8IZQ1 -
Positive control
- ICC/IF: RKO cells. WB: Rat brain lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-WDFY3 antibody - N-terminal (ab240917)Anti-WDFY3 antibody - N-terminal (ab240917) at 1/1000 dilution (1 hour at RT) + Rat brain lysate at 15 µg
Secondary
Goat Anti-Rabbit HRP for 60 min at RT at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 395 kDa
Exposure time: 6 minutesBlock: 5% Skimmed milk in 1X TBST.
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Immunocytochemistry/ Immunofluorescence - Anti-WDFY3 antibody - N-terminal (ab240917)Immunocytochemistry/Immunofluorescence analysis of RKO (human colon carcinoma cell line ) cells labeling WDFY3 using ab240917 at 1/100 dilution for 60 minutes at RT. Cells were fixed in 4% formaldehyde for 15 minutes at RT.
Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1/100 dilution for 60 minutes at RT.
Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain.
(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab240917 (D) Composite.
