Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-VPS28 antibody [EPR9880] - BSA and Azide free (ab249102)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9880] to VPS28 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-VPS28 antibody [EPR9880] - BSA and Azide free
    See all VPS28 primary antibodies

  • Description

    Rabbit monoclonal [EPR9880] to VPS28 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab249102 is the carrier-free version of ab154793. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249102 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-VPS28 antibody [EPR9880] - BSA and Azide free (ab249102)
    Western blot - Anti-VPS28 antibody [EPR9880] - BSA and Azide free (ab249102)
    Anti-VPS28 antibody [EPR9880] (ab154793) at 1/2000 dilution (unpurified) + HepG2 cell lysate at 10 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 25 kDa
    Observed band size: 25 kDa



    This data was developed using ab154793, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-VPS28 antibody [EPR9880] - BSA and Azide free (ab249102)
    Western blot - Anti-VPS28 antibody [EPR9880] - BSA and Azide free (ab249102)
    Anti-VPS28 antibody [EPR9880] (ab154793) at 1/1500 dilution (purified) + HepG2 cell lysate at 10 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 25 kDa
    Observed band size: 25 kDa



    This data was developed using ab154793, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-VPS28 antibody [EPR9880] - BSA and Azide free (ab249102)
    Western blot - Anti-VPS28 antibody [EPR9880] - BSA and Azide free (ab249102)
    All lanes : Anti-VPS28 antibody [EPR9880] (ab154793) at 1/2000 dilution (unpurified)

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Rat brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 25 kDa
    Observed band size: 25 kDa



    This data was developed using ab154793, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-VPS28 antibody [EPR9880] - BSA and Azide free (ab249102)
    Western blot - Anti-VPS28 antibody [EPR9880] - BSA and Azide free (ab249102)
    All lanes : Anti-VPS28 antibody [EPR9880] (ab154793) at 1/1500 dilution (purified)

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Rat brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 25 kDa
    Observed band size: 25 kDa



    This data was developed using ab154793, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-VPS28 antibody [EPR9880] - BSA and Azide free (ab249102)
    Western blot - Anti-VPS28 antibody [EPR9880] - BSA and Azide free (ab249102)
    All lanes : Anti-VPS28 antibody [EPR9880] (ab154793) at 1/1000 dilution (unpurified)

    Lane 1 : HepG2 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : LnCaP cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 25 kDa



    This data was developed using ab154793, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-VPS28 antibody [EPR9880] - BSA and Azide free (ab249102)
    Immunoprecipitation - Anti-VPS28 antibody [EPR9880] - BSA and Azide free (ab249102)

    This data was developed using ab154793, the same antibody clone in a different buffer formulation.

    ab154793 (unpurified) at 1/50 immunoprecipitating VPS28 in jurkat cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-VPS28 antibody [EPR9880] - BSA and Azide free (ab249102)
    Immunoprecipitation - Anti-VPS28 antibody [EPR9880] - BSA and Azide free (ab249102)

    This data was developed using ab154793, the same antibody clone in a different buffer formulation.

    ab154793 (purified) at 1/50 immunoprecipitating VPS28 in jurkat cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-VPS28 antibody [EPR9880] - BSA and Azide free (ab249102)
    Anti-VPS28 antibody [EPR9880] - BSA and Azide free (ab249102)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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