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Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free (ab273655)

Key features and details

  • Mouse monoclonal [OTI1E1] to Von Hippel Lindau/VHL - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG2b

Overview

  • Product name

    Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free
    See all Von Hippel Lindau/VHL primary antibodies

  • Description

    Mouse monoclonal [OTI1E1] to Von Hippel Lindau/VHL - BSA and Azide free

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein aa 1 to the C-terminus. Produced in HEK-293T cells.
    Database link: P40337

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: pCMV6-ENTRY VHL transfected HEK-293T cell lsyate. IHC-P: Human colon carcinoma, ovary adenocarcinoma, pancreas, endometrium adenocarcinoma, lung carcinoma, endometrium and bladder tissue.

  • General notes

    ab273655 is the carrier free version of ab140989.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free (ab273655)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free (ab273655)

    Paraffin-embedded human pancreas tissue stained for Von Hippel Lindau/VHL using ab140989 at a 1/150 dilution in immunohistochemical analysis.

    Heat-induced epitope retrieval was performed using 10 mM citric buffer, pH 6.0, 120°C for 3 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab140989).

  • Western blot - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free (ab273655)
    Western blot - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free (ab273655)
    All lanes : Anti-Von Hippel Lindau/VHL antibody [OTI1E1] (ab140989) at 1/4000 dilution

    Lane 1 : HEK-293T cells were transfected with the pCMV6-ENTRY control cDNA
    Lane 2 : HEK-293T cells were transfected with the pCMV6-ENTRY VHL cDNA

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 24 kDa



    Cells were transfected with cDNA for 48 hours then lysed.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab140989).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free (ab273655)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free (ab273655)

    Paraffin-embedded human ovary adenocarcinoma tissue stained for Von Hippel Lindau/VHL using ab140989 at a 1/150 dilution in immunohistochemical analysis.

    Heat-induced epitope retrieval was performed using 10 mM citric buffer, pH 6.0, 120°C for 3 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab140989).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free (ab273655)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free (ab273655)

    Paraffin-embedded human lung carcinoma tissue stained for Von Hippel Lindau/VHL using ab140989 at a 1/150 dilution in immunohistochemical analysis.

    Heat-induced epitope retrieval was performed using 10 mM citric buffer, pH 6.0, 120°C for 3 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab140989).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free (ab273655)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free (ab273655)

    Paraffin-embedded human endometrium tissue stained for Von Hippel Lindau/VHL using ab140989 at a 1/150 dilution in immunohistochemical analysis.

    Heat-induced epitope retrieval was performed using 10 mM citric buffer, pH 6.0, 120°C for 3 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab140989).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free (ab273655)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free (ab273655)

    Paraffin-embedded human endometrium adenocarcinoma tissue stained for Von Hippel Lindau/VHL using ab140989 at a 1/150 dilution in immunohistochemical analysis.

    Heat-induced epitope retrieval was performed using 10 mM citric buffer, pH 6.0, 120°C for 3 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab140989).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free (ab273655)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free (ab273655)

    Paraffin-embedded human bladder tissue stained for Von Hippel Lindau/VHL using ab140989 at a 1/150 dilution in immunohistochemical analysis.

    Heat-induced epitope retrieval was performed using 10 mM citric buffer, pH 6.0, 120°C for 3 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab140989).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free (ab273655)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Hippel Lindau/VHL antibody [OTI1E1] - BSA and Azide free (ab273655)

    Paraffin-embedded human colon adenocarcinoma tissue stained for Von Hippel Lindau/VHL using ab140989 at a 1/150 dilution in immunohistochemical analysis.

    Heat-induced epitope retrieval was performed using 10 mM citric buffer, pH 6.0, 120°C for 3 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab140989).

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