Anti-VLK antibody (ab229077)
Key features and details
- Rabbit polyclonal to VLK
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-VLK antibody -
Description
Rabbit polyclonal to VLK -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow
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Immunogen
Recombinant fragment within Human VLK. The exact sequence is proprietary.
Database link: Q504Y2 -
Positive control
- WB: HEK-293T whole cell lysate. IHC-P: Human colon carcinoma tissue. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-VLK antibody (ab229077)
Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for VLK (green) using ab229077 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
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Western blot - Anti-VLK antibody (ab229077)Anti-VLK antibody (ab229077) at 1/5000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 54 kDa7.5% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VLK antibody (ab229077)Paraffin-embedded human colon carcinoma tissue stained for VLK using ab229077 at 1/500 dilution in immunohistochemical analysis.