Recombinant human IGF1 Receptor protein (ab155622)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, ELISA, SDS-PAGE
Preparation and Storage
-
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 95% PBS, 5% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
-
ReconstitutionIt is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 400 ug/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.
Images
-
SDS-PAGE - Recombinant human IGF1 Receptor protein (ab155622)Human IGF-I R on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
-
Functional Studies - Recombinant human IGF1 Receptor protein (ab155622)Immobilized Human IGF-I R at 5 µg/mL (100 µL/well) can bind Biotinylated Human IGF-II, Fc Tag with a linear range of 0.156-5 µg/mL. -
Functional Studies - Recombinant human IGF1 Receptor protein (ab155622)Immobilized Human IGF-I R at 5 µg/mL (100 µL/well) can bind Human IGF-I, Fc Tag with a linear range of 0.04-0.313 µg/mL. -
SDS-PAGE - Recombinant human IGF1 Receptor protein (ab155622)SDS-PAGE analysis of reduced ab155622 stained overnight with Coomassie Blue.
