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Anti-Vitamin B2 antibody [EPR20853-141] - BSA and Azide free (ab254211)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20853-141] to Vitamin B2 - BSA and Azide free
  • Suitable for: Dot blot

Overview

  • Product name

    Anti-Vitamin B2 antibody [EPR20853-141] - BSA and Azide free
    See all Vitamin B2 primary antibodies

  • Description

    Rabbit monoclonal [EPR20853-141] to Vitamin B2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Dot blotmore details

  • Immunogen

    Chemical/ Small Molecule corresponding to Vitamin B2 conjugated to bovine serum albumin.

  • Positive control

    • Dot blot: Ovalbumin (OVA)-Riboflavin.

  • General notes

    Ab254211 is the carrier-free version of ab218125. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab254211 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Dot Blot - Anti-Vitamin B2 antibody [EPR20853-141] - BSA and Azide free (ab254211)
    Dot Blot - Anti-Vitamin B2 antibody [EPR20853-141] - BSA and Azide free (ab254211)

    Dot blot analysis of Vitamin B12 using ab218125 at 1/1000 dilution. Secondary antibody was Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100,000 dilution.

    Lane 1: Ovalbumin (OVA)-Riboflavin

    Lane 2: OVA-Folic acid (carboxyl conjugation)

    Lane 3: OVA-Folic acid (amino conjugation)

    Lane 4: OVA-Histamine

    Exposure time: 3 minutes.

    Blocking/Diluting buffer: 5% NFDM/TBST 

    Folic acid and Histamine are the analogue of Riboflavin.

    Ovalbumin (OVA)-conjugated small molecules are kindly provided by Dr Rao Yi.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218125).

  • Anti-Vitamin B2 antibody [EPR20853-141] - BSA and Azide free (ab254211)
    Anti-Vitamin B2 antibody [EPR20853-141] - BSA and Azide free (ab254211)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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