Anti-VCIP 135 antibody (ab241946)
Key features and details
- Rabbit polyclonal to VCIP 135
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-VCIP 135 antibody
See all VCIP 135 primary antibodies -
Description
Rabbit polyclonal to VCIP 135 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human VCIP 135 aa 1100-1150. The exact sequence is proprietary. NP_079330.2
Database link: Q96JH7 -
Positive control
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate. IHC-P: Human breast carcinoma and ovarian carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to VCIP 135 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for VCIP 135 with ab241946 at 1/200 dilution in immunohistochemical analysis.
Detection: DAB staining.
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Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for VCIP 135 with ab241946 at 1/200 dilution in immunohistochemical analysis.
Detection: DAB staining.
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VCIP 135 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).
ab241946 used for IP at 3 µg/mg lysate. For WB at 1 µg/ml.
Lane 1: ab241946 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Chemiluminescence detection: 3 seconds.
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All lanes : Anti-VCIP 135 antibody (ab241946) at 0.04 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 134 kDa
Exposure time: 3 minutes