Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Vaspin antibody [EPR23079-196] - BSA and Azide free (ab273096)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23079-196] to Vaspin - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse

Overview

  • Product name

    Anti-Vaspin antibody [EPR23079-196] - BSA and Azide free
    See all Vaspin primary antibodies

  • Description

    Rabbit monoclonal [EPR23079-196] to Vaspin - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse skin and stomach tissue lysates; His-tagged recombinant mouse Vaspin protein (aa 20-413). IP: Mouse stomach tissue lysate.

  • General notes

    This antibody (Clone EPR23079-196) is a different clone to ab267471 (Clone EPR23080-232).

    ab273096 is the carrier-free version of ab267470. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-Vaspin antibody [EPR23079-196] - BSA and Azide free (ab273096)
    Immunoprecipitation - Anti-Vaspin antibody [EPR23079-196] - BSA and Azide free (ab273096)

    Vaspin was immunoprecipitated from 0.35 mg Mouse stomach tissue lysate 10 with ab267470 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab267470 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/1000 dilution.

    Lane 1: Mouse stomach tissue lysate 10 ug

    Lane 2: ab267470 IP in Mouse stomach tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab267470 in mouse stomach tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 24 seconds

    Vaspin protein can form polymers when heated as described in the literature (PMID: 28668641).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab267470).

  • Anti-Vaspin antibody [EPR23079-196] - BSA and Azide free (ab273096)
    Anti-Vaspin antibody [EPR23079-196] - BSA and Azide free (ab273096)

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