Anti-Vaspin antibody [EPR23079-196] - BSA and Azide free (ab273096)
![Anti-Vaspin antibody [EPR23079-196] - BSA and Azide free (ab273096)](https://www.abcam.com/ps/products/273/ab273096/Images/ab273096-1-anti-vaspin-antibody-epr23079196-immunoprecipitation-mouse-stomach-tissue-lysate-10-ug.jpg "Anti-Vaspin antibody [EPR23079-196] - BSA and Azide free (ab273096)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23079-196] to Vaspin - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse
Overview
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Product name
Anti-Vaspin antibody [EPR23079-196] - BSA and Azide free
See all Vaspin primary antibodies -
Description
Rabbit monoclonal [EPR23079-196] to Vaspin - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse skin and stomach tissue lysates; His-tagged recombinant mouse Vaspin protein (aa 20-413). IP: Mouse stomach tissue lysate.
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General notes
This antibody (Clone EPR23079-196) is a different clone to ab267471 (Clone EPR23080-232).
ab273096 is the carrier-free version of ab267470. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23079-196 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-Vaspin antibody [EPR23079-196] - BSA and Azide free (ab273096)
Vaspin was immunoprecipitated from 0.35 mg Mouse stomach tissue lysate 10 with ab267470 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab267470 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/1000 dilution.
Lane 1: Mouse stomach tissue lysate 10 ug
Lane 2: ab267470 IP in Mouse stomach tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab267470 in mouse stomach tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 24 seconds
Vaspin protein can form polymers when heated as described in the literature (PMID: 28668641).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab267470).
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![Anti-Vaspin antibody [EPR23079-196] - BSA and Azide free (ab273096)](https://www.abcam.com/ps/products/273/ab273096/Images/ab273096-2-benefits-of-recombinant-antibodies.png)
Anti-Vaspin antibody [EPR23079-196] - BSA and Azide free (ab273096)
