Anti-VAP1 antibody (ab42885)
Key features and details
- Rabbit polyclonal to VAP1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-VAP1 antibody
See all VAP1 primary antibodies -
Description
Rabbit polyclonal to VAP1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Rat VAP1 aa 150-250.
(Peptide available asab42884) -
Positive control
- This antibody gave a positive signal in the following Tissue Lysates: Lung (Rat), Lung (Mouse) and Lung (Human). This antibody gave a positive result in IHC in the following FFPE tissue: Human normal lung.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab42885 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 85 kDa). Target
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Function
Cell adhesion protein that participates in lymphocyte recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion. Has a monoamine oxidase activity. May play a role in adipogenesis. -
Tissue specificity
Strongly expressed on the high endothelial venules of peripheral lymph nodes and on hepatic endothelia. Also highly expressed in appendix, lung and small intestine. Expressed also in adipose tissue, in bone marrow, colon, heart, kidney, ovary, pancreas, placenta, prostate, skeletal muscle, spleen and testis. -
Sequence similarities
Belongs to the copper/topaquinone oxidase family. -
Post-translational
modificationsTopaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.
N- and O-glycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 8639 Human
- Entrez Gene: 11754 Mouse
- Entrez Gene: 29473 Rat
- Omim: 603735 Human
- SwissProt: Q16853 Human
- SwissProt: O70423 Mouse
- SwissProt: O08590 Rat
- Unigene: 198241 Human
see all -
Alternative names
- Amine oxidase, copper containing 3 (vascular adhesion protein 1) antibody
- AOC3 antibody
- AOC3_HUMAN antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAP1 antibody (ab42885)
IHC image of VAP1 antibody staining in a section of formalin-fixed paraffin-embedded normal human lung* performed on a Leica BONDTM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab42885, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Western blot - Anti-VAP1 antibody (ab42885)All lanes : Anti-VAP1 antibody (ab42885) at 1 µg/ml
Lane 1 : Lung (Rat) Tissue Lysate
Lane 2 : Lung (Mouse) Whole Cell Lysate - normal tissue
Lane 3 : Lung (Human) Tissue Lysate - Fetal Normal Tissue
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 85 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa. We are unsure as to the identity of these extra bands.
VAP1 has a predicted molecular weight of 85 kDa; however it has a number of potential glycosylation sites which may affect the migration of the protein (SwissProt data). The observed 100kDa band is consistent with other commercially avaliable antibodies.
Protocols
Datasheets and documents
References (2)
ab42885 has been referenced in 2 publications.
- Romauch M Zinc-a2-glycoprotein as an inhibitor of amine oxidase copper-containing 3. Open Biol 10:190035 (2020). PubMed: 32315567
- Cha YJ et al. Site-specific expression of amine oxidases in breast cancer metastases. Tumour Biol 40:1010428318776822 (2018). PubMed: 29775160
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAP1 antibody (ab42885)
IHC image of VAP1 antibody staining in a section of formalin-fixed paraffin-embedded normal human lung* performed on a Leica BONDTM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab42885, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Western blot - Anti-VAP1 antibody (ab42885)All lanes : Anti-VAP1 antibody (ab42885) at 1 µg/ml
Lane 1 : Lung (Rat) Tissue Lysate
Lane 2 : Lung (Mouse) Whole Cell Lysate - normal tissue
Lane 3 : Lung (Human) Tissue Lysate - Fetal Normal Tissue
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 85 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa. We are unsure as to the identity of these extra bands.
VAP1 has a predicted molecular weight of 85 kDa; however it has a number of potential glycosylation sites which may affect the migration of the protein (SwissProt data). The observed 100kDa band is consistent with other commercially avaliable antibodies.
