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Microbiology Interspecies Interaction Host Virus Interaction

Anti-VAC14 antibody - C-terminal (ab213516)

Price and availability

244 579 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-VAC14 antibody - C-terminal (ab213516)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to VAC14 - C-terminal
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-VAC14 antibody - C-terminal
  • Description

    Rabbit polyclonal to VAC14 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide corresponding to Human VAC14 aa 750 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    Database link: Q08WQ2

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Signal Transduction
    • Signaling Pathway
    • Lipid Signaling
    • Other
    • Signal Transduction
    • Protein Trafficking
    • Organelle Proteins

Images

  • Western blot - Anti-VAC14 antibody - C-terminal (ab213516)
    Western blot - Anti-VAC14 antibody - C-terminal (ab213516)
    All lanes : Anti-VAC14 antibody - C-terminal (ab213516) at 0.75 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : HEK-293T cell lysate
    Lane 3 : HeLa cell lysate with Human VAC14 immunizing peptide
    Lane 4 : HEK-293T cell lysate with Human VAC14 immunizing peptide
    Lane 5 : HepG2 cell lysate
    Lane 6 : A431 cell lysate
    Lane 7 : PC12 cell lysate
    Lane 8 : Rat brain (S1 fraction)
    Lane 9 : Mouse brain (S1 fraction)

    Predicted band size: 88 kDa

  • Western blot - Anti-VAC14 antibody - C-terminal (ab213516)
    Western blot - Anti-VAC14 antibody - C-terminal (ab213516)

    VAC14 was immunoprecipitated from A431 cell lysate with ab213516.

    Western blot was performed from the immunoprecipitate using ab213516.

    Lane 1: IP with ab213516 at 10ug.

    Lane 2: IP with ab213516 at 15ug.

    Lane 3: Negative control: without ab213516.

    Lane 4: Negative control: without cell lysate.

  • Immunocytochemistry/ Immunofluorescence - Anti-VAC14 antibody - C-terminal (ab213516)
    Immunocytochemistry/ Immunofluorescence - Anti-VAC14 antibody - C-terminal (ab213516)
    Immunofluorescence analysis of HeLa cells labeling VAC14 with ab213516 at 8µg/ml, followed by Goat Anti-Rabbit IgG, Cy3 conjugate secondary detection (Red). Nuclear staining with DAPI (blue).

     

     
     
     
     
     
    Cytoplasmic and nuclear localization of VAC14 in HeLa cells. Fixed cells were stained with 8 μg/mL Anti-VAC14 (C-terminal) (Cat. No. SAB4200075) followed by Goat Anti-Rabbit IgG, Cy3 conjugate (×40 magnification). Nuclear staining with DAPI.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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