Anti-VAC14 antibody - C-terminal (ab213516)
Key features and details
- Rabbit polyclonal to VAC14 - C-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-VAC14 antibody - C-terminal -
Description
Rabbit polyclonal to VAC14 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow -
Immunogen
Synthetic peptide corresponding to Human VAC14 aa 750 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
Database link: Q08WQ2 -
General notes
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
-
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
All lanes : Anti-VAC14 antibody - C-terminal (ab213516) at 0.75 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : HEK-293T cell lysate
Lane 3 : HeLa cell lysate with Human VAC14 immunizing peptide
Lane 4 : HEK-293T cell lysate with Human VAC14 immunizing peptide
Lane 5 : HepG2 cell lysate
Lane 6 : A431 cell lysate
Lane 7 : PC12 cell lysate
Lane 8 : Rat brain (S1 fraction)
Lane 9 : Mouse brain (S1 fraction)
Predicted band size: 88 kDa
-
VAC14 was immunoprecipitated from A431 cell lysate with ab213516.
Western blot was performed from the immunoprecipitate using ab213516.
Lane 1: IP with ab213516 at 10ug.
Lane 2: IP with ab213516 at 15ug.
Lane 3: Negative control: without ab213516.
Lane 4: Negative control: without cell lysate.
-
Immunofluorescence analysis of HeLa cells labeling VAC14 with ab213516 at 8µg/ml, followed by Goat Anti-Rabbit IgG, Cy3 conjugate secondary detection (Red). Nuclear staining with DAPI (blue).