Anti-USP9Y antibody [EPR13966] - BSA and Azide free (ab250518)
![Anti-USP9Y antibody [EPR13966] - BSA and Azide free (ab250518)](https://www.abcam.com/ps/products/250/ab250518/Images/ab250518-402954-anti-usp9y-antibody-epr13966-bsa-and-azide-free-western-blot.jpg "Anti-USP9Y antibody [EPR13966] - BSA and Azide free (ab250518)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13966] to USP9Y - BSA and Azide free
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-USP9Y antibody [EPR13966] - BSA and Azide free
See all USP9Y primary antibodies -
Description
Rabbit monoclonal [EPR13966] to USP9Y - BSA and Azide free -
Host species
Rabbit -
Specificity
The immunogen of USP9Y has 78% homology with USP9X (Uniprot: Q93008), which is a homologue of USP9Y on the X chromosome. In female-derived HeLa cells, we found a band which mostly likely to be USP9X. Therefore, this antibody may cross-react with USP9X.
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Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250518 is the carrier-free version of ab181842. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250518 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13966 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-USP9Y antibody [EPR13966] - BSA and Azide free (ab250518)All lanes : Anti-USP9Y antibody [EPR13966] (ab181842) at 1/20000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Molt-4 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 291 kDa
Observed band size: 291 kDaThis data was developed using ab181842, the same antibody clone in a different buffer formulation.
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![Flow Cytometry - Anti-USP9Y antibody [EPR13966] - BSA and Azide free (ab250518)](https://www.abcam.com/ps/products/250/ab250518/Images/ab250518-1-anti-usp9y-antibody-epr13966-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-USP9Y antibody [EPR13966] - BSA and Azide free (ab250518)This data was developed using ab181842, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling USP9Y with ab181842 at 1/200 diltuion (red) compared to a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution. -
![Anti-USP9Y antibody [EPR13966] - BSA and Azide free (ab250518)](https://www.abcam.com/ps/products/250/ab250518/Images/ab250518-3-benefits-of-recombinant-antibodies.png)
Anti-USP9Y antibody [EPR13966] - BSA and Azide free (ab250518)
