Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-USP9Y antibody [EPR13966] - BSA and Azide free (ab250518)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13966] to USP9Y - BSA and Azide free
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-USP9Y antibody [EPR13966] - BSA and Azide free
    See all USP9Y primary antibodies

  • Description

    Rabbit monoclonal [EPR13966] to USP9Y - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The immunogen of USP9Y has 78% homology with USP9X (Uniprot: Q93008), which is a homologue of USP9Y on the X chromosome. In female-derived HeLa cells, we found a band which mostly likely to be USP9X. Therefore, this antibody may cross-react with USP9X.

  • Tested applications

    Suitable for: WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250518 is the carrier-free version of ab181842. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250518 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-USP9Y antibody [EPR13966] - BSA and Azide free (ab250518)
    Western blot - Anti-USP9Y antibody [EPR13966] - BSA and Azide free (ab250518)
    All lanes : Anti-USP9Y antibody [EPR13966] (ab181842) at 1/20000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : Molt-4 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 291 kDa
    Observed band size: 291 kDa



    This data was developed using ab181842, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-USP9Y antibody [EPR13966] - BSA and Azide free (ab250518)
    Flow Cytometry - Anti-USP9Y antibody [EPR13966] - BSA and Azide free (ab250518)
    This data was developed using ab181842, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling USP9Y with ab181842 at 1/200 diltuion (red) compared to a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
  • Anti-USP9Y antibody [EPR13966] - BSA and Azide free (ab250518)
    Anti-USP9Y antibody [EPR13966] - BSA and Azide free (ab250518)

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