Anti-USP26 antibody (ab101650)
Key features and details
- Rabbit polyclonal to USP26
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-USP26 antibody
See all USP26 primary antibodies -
Description
Rabbit polyclonal to USP26 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human USP26 aa 650-750 conjugated to keyhole limpet haemocyanin.
(Peptide available asab115930)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab101650 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanWB HumanApplication Abreviews Notes ICC/IF Use a concentration of 1 µg/ml.WB (1) Use a concentration of 1 µg/ml. Detects a band of approximately 94 kDa (predicted molecular weight: 104 kDa).Notes ICC/IF
Use a concentration of 1 µg/ml.WB
Use a concentration of 1 µg/ml. Detects a band of approximately 94 kDa (predicted molecular weight: 104 kDa).Target
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Function
Involved in the ubiquitin-dependent proteolytic pathway in conjunction with the 26S proteasome (By similarity). Deubiquinates the androgen receptor and regulates the androgen receptor signaling pathway. -
Sequence similarities
Belongs to the peptidase C19 family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 83844 Human
- Omim: 300309 Human
- SwissProt: Q9BXU7 Human
- Unigene: 333137 Human
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Alternative names
- Deubiquitinating enzyme 26 antibody
- MGC120066 antibody
- MGC120067 antibody
see all
Images
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Western blot - Anti-USP26 antibody (ab101650)All lanes : Anti-USP26 antibody (ab101650) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
with immunising peptide at 1 µg/ml
Lane 5 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate with immunising peptide at 1 µg/ml
Lane 6 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate with immunising peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 104 kDa
Observed band size: 94 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-USP26 antibody (ab101650)ICC/IF image of ab101650 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab101650 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References (4)
ab101650 has been referenced in 4 publications.
- Li L et al. USP26 promotes esophageal squamous cell carcinoma metastasis through stabilizing Snail. Cancer Lett 448:52-60 (2019). PubMed: 30763716
- Kit Leng Lui S et al. USP26 regulates TGF-ß signaling by deubiquitinating and stabilizing SMAD7. EMBO Rep 18:797-808 (2017). PubMed: 28381482
- Ning B et al. USP26 functions as a negative regulator of cellular reprogramming by stabilising PRC1 complex components. Nat Commun 8:349 (2017). PubMed: 28839133
- Wosnitzer MS et al. Ubiquitin Specific Protease 26 (USP26) Expression Analysis in Human Testicular and Extragonadal Tissues Indicates Diverse Action of USP26 in Cell Differentiation and Tumorigenesis. PLoS One 9:e98638 (2014). IHC-P, IHC-Fr ; Human . PubMed: 24922532
Images
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Western blot - Anti-USP26 antibody (ab101650)All lanes : Anti-USP26 antibody (ab101650) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
with immunising peptide at 1 µg/ml
Lane 5 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate with immunising peptide at 1 µg/ml
Lane 6 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate with immunising peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 104 kDa
Observed band size: 94 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes
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Immunocytochemistry/ Immunofluorescence - Anti-USP26 antibody (ab101650)
ICC/IF image of ab101650 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab101650 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
