Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-USP19 antibody [EPR14816] (ab189518)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14816] to USP19
  • Suitable for: WB, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-USP19 antibody [EPR14816]
    See all USP19 primary antibodies

  • Description

    Rabbit monoclonal [EPR14816] to USP19

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Jurkat, 293T and HeLa lysates. Human fetal brain lysate. Jurkat cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-USP19 antibody [EPR14816] (ab189518)
    Western blot - Anti-USP19 antibody [EPR14816] (ab189518)

    Lane 1: Wild-type HAP1 cell lysate (40 µg)
    Lane 2: USP19 knockout HAP1 cell lysate (40 µg)
    Lane 3: Jurkat cell lysate (40 µg)
    Lane 4: HeLa cell lysate (40 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab189518 observed at 160 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab189518 was shown to recognize USP19  when USP19  knockout samples were used, along with additional cross-reactive bands. Wild-type and USP19  knockout samples were subjected to SDS-PAGE. Ab189518 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-USP19 antibody [EPR14816] (ab189518)
    Flow Cytometry - Anti-USP19 antibody [EPR14816] (ab189518)

    Flow cytometric analysis of Jurkat cells (left histogram) labeling USP19 with ab189518 at 1/250 dilution compared to negative control cells (right histogram). Secondary antibody FITC-conjugated goat-anti-rabbit IgG used at 1/150 diution.

  • Western blot - Anti-USP19 antibody [EPR14816] (ab189518)
    Western blot - Anti-USP19 antibody [EPR14816] (ab189518)
    Anti-USP19 antibody [EPR14816] (ab189518) at 1/5000 dilution + Human fetal brain lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 146 kDa

  • Western blot - Anti-USP19 antibody [EPR14816] (ab189518)
    Western blot - Anti-USP19 antibody [EPR14816] (ab189518)
    All lanes : Anti-USP19 antibody [EPR14816] (ab189518) at 1/10000 dilution

    Lane 1 : Jurkat lysate
    Lane 2 : 293T lysate
    Lane 3 : HeLa lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 146 kDa

  • Anti-USP19 antibody [EPR14816] (ab189518)
    Anti-USP19 antibody [EPR14816] (ab189518)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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