Antibodies, Proteins, Kits and Reagents for Life Science

331 689 ₸ Product size

100 µl 331 689 ₸

Order now and get it on Tuesday March 02, 2021

Anti-USP19 antibody [EPR14816] (ab189518)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14816] to USP19
Suitable for: WB, Flow Cyt
Knockout validated
Reacts with: Human

Product name

Anti-USP19 antibody [EPR14816]
See all USP19 primary antibodies
Description

Rabbit monoclonal [EPR14816] to USP19
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Human

WB
Human

See all applications and species data
Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- Jurkat, 293T and HeLa lysates. Human fetal brain lysate. Jurkat cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
Flow Cyt	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14816
Isotype

IgG
Research areas

Western blot - Anti-USP19 antibody [EPR14816] (ab189518)

Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: USP19 knockout HAP1 cell lysate (40 µg)
Lane 3: Jurkat cell lysate (40 µg)
Lane 4: HeLa cell lysate (40 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab189518 observed at 160 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab189518 was shown to recognize USP19 when USP19 knockout samples were used, along with additional cross-reactive bands. Wild-type and USP19 knockout samples were subjected to SDS-PAGE. Ab189518 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Flow Cytometry - Anti-USP19 antibody [EPR14816] (ab189518)

Flow cytometric analysis of Jurkat cells (left histogram) labeling USP19 with ab189518 at 1/250 dilution compared to negative control cells (right histogram). Secondary antibody FITC-conjugated goat-anti-rabbit IgG used at 1/150 diution.
Western blot - Anti-USP19 antibody [EPR14816] (ab189518)

Anti-USP19 antibody [EPR14816] (ab189518) at 1/5000 dilution + Human fetal brain lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 146 kDa
Western blot - Anti-USP19 antibody [EPR14816] (ab189518)

All lanes : Anti-USP19 antibody [EPR14816] (ab189518) at 1/10000 dilution

Lane 1 : Jurkat lysate
Lane 2 : 293T lysate
Lane 3 : HeLa lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 146 kDa
Anti-USP19 antibody [EPR14816] (ab189518)

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