Anti-USO1 antibody (ab184014)
Key features and details
- Rabbit polyclonal to USO1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-USO1 antibody
See all USO1 primary antibodies -
Description
Rabbit polyclonal to USO1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment within Human USO1 aa 717-962. The exact sequence is proprietary.
Database link: O60763 -
Positive control
- WB: Neuro2A, C8D30, NIH-3T3, Raw264.7, C2C12, HEK-293T, A431 , HeLa , HepG2 whole cell lysates, IF: A431 cells.
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General notes
Previously labelled as Vesicle docking protein p115.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-USO1 antibody (ab184014)All lanes : Anti-USO1 antibody (ab184014) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 108 kDa7.5% SDS PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-USO1 antibody (ab184014)Immunofluorescence analysis of methanol fixed A431 (human epidermoid carcinoma cell line) cells labelling USO1 in the Golgi apparatus with ab184014 at 1/500 dilution (green) and counterstained with Hoechst 33342. Cells were fixed in 100% methanol for 5 mins.
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Western blot - Anti-USO1 antibody (ab184014)All lanes : Anti-USO1 antibody (ab184014) at 1/1000 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate
Lane 2 : C8-D30 (mouse astrocyte type III cerebellum cell line) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 4 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 5 : C2C12 (mouse myoblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 108 kDa5% SDS-PAGE
