Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Uroplakin II/UPII antibody [EPR18799] - BSA and Azide free (ab224261)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18799] to Uroplakin II/UPII - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Uroplakin II/UPII antibody [EPR18799] - BSA and Azide free
    See all Uroplakin II/UPII primary antibodies

  • Description

    Rabbit monoclonal [EPR18799] to Uroplakin II/UPII - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human bladder, ureter and bladder cancer tissues.

  • General notes

    ab224261 is the carrier-free version of ab213655 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab224261 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Previously labelled as Uroplakin II.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR18799

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin II/UPII antibody [EPR18799] - BSA and Azide free (ab224261)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin II/UPII antibody [EPR18799] - BSA and Azide free (ab224261)

    Immunohistochemical analysis of paraffin-embedded human ureter tissue labeling Uroplakin II/UPII with ab213655 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic and weak membrane staining on epithelial cells of human ureter tissues is observed [PMID:24978921]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213655).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin II/UPII antibody [EPR18799] - BSA and Azide free (ab224261)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin II/UPII antibody [EPR18799] - BSA and Azide free (ab224261)

    Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling Uroplakin II/UPII with ab213655 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane and weak cytoplasmic staining on tumor cells of human bladder cancer is observed [PMID:24978921]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213655).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin II/UPII antibody [EPR18799] - BSA and Azide free (ab224261)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin II/UPII antibody [EPR18799] - BSA and Azide free (ab224261)

    Immunohistochemical analysis of paraffin-embedded human renal clear cell carcinoma tissue labeling Uroplakin II/UPII with ab213655 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative control: No staining on tumor cells of human renal clear cell carcinoma. [PMID:24978921].

    Counter stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213655).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin II/UPII antibody [EPR18799] - BSA and Azide free (ab224261)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Uroplakin II/UPII antibody [EPR18799] - BSA and Azide free (ab224261)

    This IHC data was generated using the same anti-Uroplakin II/UPII antibody clone [EPR18799] in a different buffer formulation (cat# ab213655).

    Immunohistochemical analysis of paraffin-embedded human bladder tissue labeling Uroplakin II/UPII with ab213655 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane staining on epithelial cells of human bladder tissues is observed [PMID:24978921]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Uroplakin II/UPII antibody [EPR18799] - BSA and Azide free (ab224261)
    Anti-Uroplakin II/UPII antibody [EPR18799] - BSA and Azide free (ab224261)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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