Anti-Unrip antibody (ab205015)
Key features and details
- Rabbit polyclonal to Unrip
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-Unrip antibody
See all Unrip primary antibodies -
Description
Rabbit polyclonal to Unrip -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB MouseHuman
-
Immunogen
Synthetic peptide within Human Unrip aa 300 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_009109.3.
Database link: Q9Y3F4 -
Positive control
- HeLa, 293T, Jurkat, TCMK1 and NIH 3T3 whole cell lysates.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab205015 was affinity purified using an epitope specific to Unrip immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunoprecipitation - Anti-Unrip antibody (ab205015)
Detection of Unrip in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab205015 at 6 µg/mg lysate for IP (Lane 1). For WB detection ab205015 was used at 0.4 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds. Lysate prepared using NETN lysis buffer.
-
Western blot - Anti-Unrip antibody (ab205015)All lanes : Anti-Unrip antibody (ab205015) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK1 whole cell lysate
Lane 5 : NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 38 kDa
Exposure time: 10 secondsLysates prepared using NETN lysis buffer
