Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-UHRF1 antibody [EPR18803] - BSA and Azide free (ab251181)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18803] to UHRF1 - BSA and Azide free
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-UHRF1 antibody [EPR18803] - BSA and Azide free
    See all UHRF1 primary antibodies

  • Description

    Rabbit monoclonal [EPR18803] to UHRF1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251181 is the carrier-free version of ab194236. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251181 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-UHRF1 antibody [EPR18803] - BSA and Azide free (ab251181)
    Western blot - Anti-UHRF1 antibody [EPR18803] - BSA and Azide free (ab251181)
    All lanes : Anti-UHRF1 antibody [EPR18803] (ab194236) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 90 kDa
    Observed band size: 97 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    This data was developed using ab194236, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 12530060).

  • Western blot - Anti-UHRF1 antibody [EPR18803] - BSA and Azide free (ab251181)
    Western blot - Anti-UHRF1 antibody [EPR18803] - BSA and Azide free (ab251181)
    All lanes : Anti-UHRF1 antibody [EPR18803] (ab194236) at 1/1000 dilution

    Lane 1 : Human colon lysate
    Lane 2 : Human breast lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 90 kDa
    Observed band size: 97 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab194236, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 12530060).

  • Western blot - Anti-UHRF1 antibody [EPR18803] - BSA and Azide free (ab251181)
    Western blot - Anti-UHRF1 antibody [EPR18803] - BSA and Azide free (ab251181)
    All lanes : Anti-UHRF1 antibody [EPR18803] (ab194236) at 1/5000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
    Lane 3 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 90 kDa
    Observed band size: 97 kDa why is the actual band size different from the predicted?



    This data was developed using ab194236, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-2: 10 seconds; Lane 3: 3 minutes.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 12530060).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody [EPR18803] - BSA and Azide free (ab251181)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody [EPR18803] - BSA and Azide free (ab251181)
    This data was developed using ab194236, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling UHRF1 with ab194236 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on lymphocytes of Human tonsil is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody [EPR18803] - BSA and Azide free (ab251181)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody [EPR18803] - BSA and Azide free (ab251181)
    This data was developed using ab194236, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling UHRF1 with ab194236 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on the epithelial cells of Human colon is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody [EPR18803] - BSA and Azide free (ab251181)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody [EPR18803] - BSA and Azide free (ab251181)
    This data was developed using ab194236, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling UHRF1 with ab194236 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on lymphocytes of rat spleen is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunoprecipitation - Anti-UHRF1 antibody [EPR18803] - BSA and Azide free (ab251181)
    Immunoprecipitation - Anti-UHRF1 antibody [EPR18803] - BSA and Azide free (ab251181)

    This data was developed using ab194236, the same antibody clone in a different buffer formulation.

    UHRF1 was immunoprecipitated from 1mg of HepG2 (Human liver hepatocellular carcinoma) whole cell lysate with ab194236 at 1/20 dilution.

     Western blot was performed from the immunoprecipitate using ab194236 at 1/1000 dilution.

     VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: HepG2 whole cell lysate 10ug (Input).

    Lane 2: ab194236 IP in HepG2 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab194236 in HepG2 whole cell lysate.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 30 seconds.

  • Anti-UHRF1 antibody [EPR18803] - BSA and Azide free (ab251181)
    Anti-UHRF1 antibody [EPR18803] - BSA and Azide free (ab251181)

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