Anti-UFL1 antibody - C-terminal (ab226216)
Key features and details
- Rabbit polyclonal to UFL1 - C-terminal
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-UFL1 antibody - C-terminal
See all UFL1 primary antibodies -
Description
Rabbit polyclonal to UFL1 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rabbit, Horse, Cow, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla
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Immunogen
Synthetic peptide within Human UFL1 aa 744-794 (C terminal). The exact sequence is proprietary. (NP_056138.1).
Database link: O94874-1 -
Positive control
- WB: HeLa, HEK-293T, Jurkat and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate (ab150035).
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General notes
This product was previously labelled as K0776
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226216 was affinity purified using an epitope specific to UFL1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-UFL1 antibody - C-terminal (ab226216)All lanes : Anti-UFL1 antibody - C-terminal (ab226216) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 90 kDa
Exposure time: 30 seconds
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Immunoprecipitation - Anti-UFL1 antibody - C-terminal (ab226216)UFL1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226216 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226216 at 0.4 µg/ml.
Lane 1: ab226216 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds
