Anti-UBE2S antibody (ab177508)
Key features and details
- Rabbit polyclonal to UBE2S
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-UBE2S antibody
See all UBE2S primary antibodies -
Description
Rabbit polyclonal to UBE2S -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rabbit, Cow, Dog, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis -
Immunogen
Synthetic peptide within Human UBE2S aa 172-222. The exact sequence is proprietary. (NP_055316.2)
Sequence:ASGTEASST DPGAPGGPGG AEGPMAKKHA GERDKKLAAK KKTDKKRALR RL
Database link: Q16763 -
Positive control
- 293T, HeLa, Jurkat, mouse TCMK1 and mouse NIH 3T3 whole cell lysates.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 - 8. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab177508 was affinity purified using an epitope specific to UBE2S immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-UBE2S antibody (ab177508) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : mouse TCMK1 whole cell lysate
Lane 5 : mouse NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 24 kDa
Exposure time: 3 minutes
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Detection of UBE2S in Immunoprecipitates of 293Twhole cell lysates (1 mg for IP, 20% of IP loaded) using ab177508 at 6 µg/mg lysate for IP (Lane 1). For WB detection, ab177508 was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.