Anti-U1A antibody [EPR10632] - BSA and Azide free (ab249374)
![Anti-U1A antibody [EPR10632] - BSA and Azide free (ab249374)](https://www.abcam.com/ps/products/249/ab249374/Images/ab249374-405810-anti-u1a-antibody-epr10632-bsa-and-azide-free-western-blot.jpg "Anti-U1A antibody [EPR10632] - BSA and Azide free (ab249374)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10632] to U1A - BSA and Azide free
- Suitable for: WB, ICC, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-U1A antibody [EPR10632] - BSA and Azide free
See all U1A primary antibodies -
Description
Rabbit monoclonal [EPR10632] to U1A - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC, IPmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab249374 is the carrier-free version of ab166890. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab249374 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR10632 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-U1A antibody [EPR10632] - BSA and Azide free (ab249374)All lanes : Anti-U1A antibody [EPR10632] (ab166890) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 31 kDaThis data was developed using ab166890, the same antibody clone in a different buffer formulation.
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![Immunoprecipitation - Anti-U1A antibody [EPR10632] - BSA and Azide free (ab249374)](https://www.abcam.com/ps/products/249/ab249374/Images/ab249374-2-anti-u1a-antibody-epr10632-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-U1A antibody [EPR10632] - BSA and Azide free (ab249374)This data was developed using ab166890, the same antibody clone in a different buffer formulation.Western blot analysis (using ab166890 at 1/10000 dilution) of pellet from Jurkat cell lysate immunoprecipitated using ab166890 at 1/10000 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG. -
![Immunocytochemistry - Anti-U1A antibody [EPR10632] - BSA and Azide free (ab249374)](https://www.abcam.com/ps/products/249/ab249374/Images/ab249374-1-anti-u1a-antibody-epr10632-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-U1A antibody [EPR10632] - BSA and Azide free (ab249374) -
![Anti-U1A antibody [EPR10632] - BSA and Azide free (ab249374)](https://www.abcam.com/ps/products/249/ab249374/Images/ab249374-4-benefits-of-recombinant-antibodies.png)
Anti-U1A antibody [EPR10632] - BSA and Azide free (ab249374)
