Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TVA antibody [PICA187E] - BSA and Azide free (ab271302)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [PICA187E] to TVA - BSA and Azide free
  • Suitable for: Flow Cyt, ICC, IHC-P, WB
  • Reacts with: Bird

Overview

  • Product name

    Anti-TVA antibody [PICA187E] - BSA and Azide free
    See all TVA primary antibodies

  • Description

    Rat monoclonal [PICA187E] to TVA - BSA and Azide free

  • Host species

    Rat

  • Tested applications

    Suitable for: Flow Cyt, ICC, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Bird

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T transfected with TVA expression vector containing a myc-His-tag®, whole cell lysate. IHC-P: HEK-293T transfected with a TVA construct. Flow Cyt: HEK-293T transfected with myc tagged TVA construct cells. ICC: HEK-293T transfected with myc tagged TVA construct cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab271302 is the carrier-free version of ab271292. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab271302 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    PICA187E

  • Isotype

    IgG2b

Images

  • Western blot - Anti-TVA antibody [PICA187E] - BSA and Azide free (ab271302)
    Western blot - Anti-TVA antibody [PICA187E] - BSA and Azide free (ab271302)
    All lanes : Anti-TVA antibody [PICA187E] (ab271292) at 1/5000 dilution

    Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
    Lane 2 : HEK-293T transfected with TVA expression vector containing a myc-His-tag®, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/50000 dilution

    Observed band size: 25-37 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    This data was developed using ab271292 the same antibody clone in a different buffer formulation.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TVA antibody [PICA187E] - BSA and Azide free (ab271302)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TVA antibody [PICA187E] - BSA and Azide free (ab271302)

    This data was developed using ab271292 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded (Panel A) HEK-293T transfected with a TVA construct and (Panel B) HEK-293T transfected with empty plasmid, labeling TVA with ab271292 at 1/100 (10.98 µg/ml) dilution followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Positive staining on (Panel A) HEK-293T transfected with a TVA construct, no staining on (Panel B) HEK-293T transfected with empty plasmid. The section was incubated with ab271292 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunocytochemistry - Anti-TVA antibody [PICA187E] - BSA and Azide free (ab271302)
    Immunocytochemistry - Anti-TVA antibody [PICA187E] - BSA and Azide free (ab271302)

    This data was developed using ab271292 the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney) cells transfected with myc-tagged TVA expression vector labelling TVA with ab271292 at 1/50 (21.96 µg/ml) dilution, followed by ab150157 Goat Anti-rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HEK-293T cells transfected with myc-tagged TVA expression vector. Myc-Tag mouse mAb (Alexa Fluor® 647) was used as a counterstain at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Used PBS instead of ab271292, followed by ab150157 Goat Anti-rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution.

     

  • Flow Cytometry - Anti-TVA antibody [PICA187E] - BSA and Azide free (ab271302)
    Flow Cytometry - Anti-TVA antibody [PICA187E] - BSA and Azide free (ab271302)

    This data was developed using ab271292 the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEH-293T (human embryonic kidney epithelial cell) (transfected with myc tagged TVA construct) cells labelling TVA with ab271292 at 1/1000 dilution (0.1µg) (Right panel) compared with a rat monoclonal IgG isotype control (Left panel). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) was used as the secondary antibody at a 1/2000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TVA antibody [PICA187E] - BSA and Azide free (ab271302)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TVA antibody [PICA187E] - BSA and Azide free (ab271302)

    This data was developed using ab271292 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling TVA with ab271292 at 1/100 (10.98 µg/ml) dilution followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Negative control: No staining on human cerebrum. The section was incubated with ab271292 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TVA antibody [PICA187E] - BSA and Azide free (ab271302)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TVA antibody [PICA187E] - BSA and Azide free (ab271302)

    This data was developed using ab271292 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling TVA with ab271292 at 1/100 (10.98 µg/ml) dilution followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Negative control: No staining on mouse cerebrum. The section was incubated with ab271292 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TVA antibody [PICA187E] - BSA and Azide free (ab271302)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TVA antibody [PICA187E] - BSA and Azide free (ab271302)

    This data was developed using ab271292 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling TVA with abab271292 at 1/100 (10.98 µg/ml) dilution followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Negative control: No staining on rat cerebrum. The section was incubated with ab271292 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-TVA antibody [PICA187E] - BSA and Azide free (ab271302)
    Anti-TVA antibody [PICA187E] - BSA and Azide free (ab271302)

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