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Epigenetics and Nuclear Signaling DNA / RNA Translation tRNAs

Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424] (ab92733)

Price and availability

304 886 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424] (ab92733)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3424] to Tryptophanyl tRNA synthetase/WRS
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

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Overview

  • Product name

    Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424]
    See all Tryptophanyl tRNA synthetase/WRS primary antibodies
  • Description

    Rabbit monoclonal [EPR3424] to Tryptophanyl tRNA synthetase/WRS
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human Tryptophanyl tRNA synthetase/WRS aa 450 to the C-terminus (C terminal). The exact sequence is proprietary.

  • Positive control

    • JAR and 293T cell lysates; HeLa cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Previously labelled as Tryptophanyl tRNA synthetase. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR3424
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • tRNAs
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • tRNA synthetase

Images

  • Western blot - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424] (ab92733)
    Western blot - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424] (ab92733)
    All lanes : Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424] (ab92733) at 1/10000 dilution

    Lane 1 : JAR cell lysate
    Lane 2 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 53 kDa
    Observed band size: 53 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424] (ab92733)
    Immunocytochemistry/ Immunofluorescence - Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424] (ab92733)

    Immunofluorescence staining of MCF7 cells with purified ab92733 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 100% methanol. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.

  • Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424] (ab92733)
    Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424] (ab92733)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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