Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424] (ab92733)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3424] to Tryptophanyl tRNA synthetase/WRS
- Suitable for: WB, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424]
See all Tryptophanyl tRNA synthetase/WRS primary antibodies -
Description
Rabbit monoclonal [EPR3424] to Tryptophanyl tRNA synthetase/WRS -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human -
Immunogen
Synthetic peptide within Human Tryptophanyl tRNA synthetase/WRS aa 450 to the C-terminus (C terminal). The exact sequence is proprietary.
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Positive control
- JAR and 293T cell lysates; HeLa cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Previously labelled as Tryptophanyl tRNA synthetase.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3424 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Tryptophanyl tRNA synthetase/WRS antibody [EPR3424] (ab92733) at 1/10000 dilution
Lane 1 : JAR cell lysate
Lane 2 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 53 kDa
Observed band size: 53 kDa
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Immunofluorescence staining of MCF7 cells with purified ab92733 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 100% methanol. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.
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