Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TRPV1 antibody [BS397] - C-terminal (ab203103)

Key features and details

  • Mouse monoclonal [BS397] to TRPV1 - C-terminal
  • Suitable for: WB, IHC-Fr, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat
  • Isotype: IgG2b

Overview

  • Product name

    Anti-TRPV1 antibody [BS397] - C-terminal
    See all TRPV1 primary antibodies

  • Description

    Mouse monoclonal [BS397] to TRPV1 - C-terminal

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Rat
    ICC/IF
    Rat
    IHC-Fr
    Mouse
    WB
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Synthetic peptide within Rat TRPV1 aa 800 to the C-terminus (C terminal) (Cysteine residue). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: O35433

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: PC-12 cell lysates; Mouse brain homogenate; forskolin and NGF stimulated 50B11 hybrid mouse and rat DRG cell lysates; NGF-stimulated PC-12 cell lysates. IHC-Fr: Mouse dorsal root ganglia. ICC/IF: C6 cells. Flow Cytometry: PC-12 cells.

Properties

Images

  • Immunohistochemistry (Frozen sections) - Anti-TRPV1 antibody [BS397] - C-terminal (ab203103)
    Immunohistochemistry (Frozen sections) - Anti-TRPV1 antibody [BS397] - C-terminal (ab203103)

    Immunohistochemical analysis of mouse dorsal root ganglia labelling TRPV1 with ab203103 at 10 µg/mL. Visualized with anti-mouse-Cy3 conjugate (red).

     

  • Western blot - Anti-TRPV1 antibody [BS397] - C-terminal (ab203103)
    Western blot - Anti-TRPV1 antibody [BS397] - C-terminal (ab203103)
    Lane 1 : Anti-TRPV1 antibody [BS397] - C-terminal (ab203103) at 2 µg/ml (overnight)
    Lane 2 : Anti-TRPV1 antibody [BS397] - C-terminal (ab203103) at 2 µg/ml ((pre-absorbed))

    All lanes : Rat PC-12 (Rat adrenal gland pheochromocytoma cell line) cell lysate

    Lysates/proteins at 80 µg per lane.

    Secondary
    All lanes : Anti-mouse-HRP at 1/6000 dilution

    Developed using the ECL technique.

    Predicted band size: 95 kDa



    SDS-PAGE: denatured and reduced

  • Immunocytochemistry/ Immunofluorescence - Anti-TRPV1 antibody [BS397] - C-terminal (ab203103)
    Immunocytochemistry/ Immunofluorescence - Anti-TRPV1 antibody [BS397] - C-terminal (ab203103)

    ab203103 staining TRPV1 in C6 (Rat glial tumor cell line) cells by ICC/IF (Immunocytochemistry/immunofluorescence).

    Cells were fixed with 4% formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% horse serum. Samples were incubated with primary antibody (2 μg/ml, panel B) for 1 hour. A CF488A-conjugated Donkey anti-mouse polyclonal was used as the secondary antibody (5 μg/ml). Nuclei were stained with Hoechst dye (panel A) and panel C shows the merged image.

  • Flow Cytometry - Anti-TRPV1 antibody [BS397] - C-terminal (ab203103)
    Flow Cytometry - Anti-TRPV1 antibody [BS397] - C-terminal (ab203103)

    Analysis of TRPV1 expression in PC-12 (Rat adrenal gland pheochromocytoma cell line) cell line by Flow Cytometry (black solid).

    Cells were fixed and permeabilized in absolute methanol (10 minutes in ice) and 0.1% Tween-20 in PBS, then blocked with 1% BSA. Incubated with primary antibody for 30 minutes at RT. Secondary antibody used is a Goat anti-mouse PE labeled secondary antibody (1:100 dilution), 20 minutes in dark at room temperature.

    Negative control: Non-specific Control IgG, clone X63 (black dashed).

  • Western blot - Anti-TRPV1 antibody [BS397] - C-terminal (ab203103)
    Western blot - Anti-TRPV1 antibody [BS397] - C-terminal (ab203103)
    All lanes : Anti-TRPV1 antibody [BS397] - C-terminal (ab203103) at 1 µg/ml

    Lane 1 : Control
    Lane 2 : Forskolin stimulated 50B11 hybrid mouse x rat DRG cell lysate
    Lane 3 : Forskolin and NGF stimulated 50B11 hybrid mouse x rat DRG cell lysate
    Lane 4 : NGF-stimulated PC-12 (Rat glial tumor cell line) cell lysate (NGF control)

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 95 kDa



    (denatured and reduced)

  • Western blot - Anti-TRPV1 antibody [BS397] - C-terminal (ab203103)
    Western blot - Anti-TRPV1 antibody [BS397] - C-terminal (ab203103)
    Lane 1 : Anti-TRPV1 antibody [BS397] - C-terminal (ab203103) at 2 µg/ml
    Lane 2 : Anti-TRPV1 antibody [BS397] - C-terminal (ab203103) at 1 µg/ml
    Lane 3 : Anti-TRPV1 antibody [BS397] - C-terminal (ab203103) at 2 µg/ml (pre-absorbed)

    All lanes : Mouse brain homogenate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Anti-mouse-HRP at 1/6000 dilution

    Developed using the ECL technique.

    Predicted band size: 95 kDa



    SDS-PAGE: denatured and reduced. ab203103 detects bands at 90-100 kDa and 180-200 kDa corresponding to TRPV1 monomer and dimer.

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