Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HCN2 antibody [S71] (ab84817)

Key features and details

  • Mouse monoclonal [S71] to HCN2
  • Suitable for: IHC-Fr, IHC-P, ICC, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-HCN2 antibody [S71]
    See all HCN2 primary antibodies

  • Description

    Mouse monoclonal [S71] to HCN2

  • Host species

    Mouse

  • Specificity

    Does not cross react with HCN1.

  • Tested Applications & Species

    Application Species
    ICC
    Human
    IHC-Fr
    Mouse
    IHC-P
    Human
    WB
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Fusion protein, corresponding to cytoplasmic C terminal amino acids 761-863 of Rat HCN2

  • Positive control

    • IHC-Fr: Mouse brain tissue; IHC-P: Human hippocampus tissue; WB: Rat brain membrane and mouse brain tissue lysates; ICC: Differentiated SH-SH5Y cells.

  • General notes

    The clone number S71 has previously been called S71-37 and N71/37. These clone numbers all refer to the same antibody clone.

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunocytochemistry - Anti-HCN2 antibody [S71] (ab84817)
    Immunocytochemistry - Anti-HCN2 antibody [S71] (ab84817)

    Immunocytochemistry analysis of differentiated SH-SY5Y (human neuroblastoma cell line from bone marrow) cells labelling HCN2 with ab84817 at 1/100 dilution followed by secondary antibody (Alexa Fluor® 488) (green). Cells were counterstained with phalloidin (Alexa Fluor® 647) (red), and with Anti-beta III Tubulin antibody (Alexa Fluor® 555) (magenta). Nuclear DNA was labelled with Hoechst (blue).

    (A) Phalloidin

    (B) Anti-beta III Tubulin antibody

    (C) HCN2 Antibody (ab84817) and secondary antibody

    (D) Hoechst

    (E) Composite

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN2 antibody [S71] (ab84817)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN2 antibody [S71] (ab84817)

    Immunohistochemistry (Bouin-fixed paraffin-embedded sections) analysis of human hippocampus tissue labelling HCN2 with ab84817 at 1/100 dilution (1 hour at room temperature). FITC Goat Anti-Mouse (green) was used as the secondary antibody at 1/50 dilution (1 hour at room temperature).

  • Western blot - Anti-HCN2 antibody [S71] (ab84817)
    Western blot - Anti-HCN2 antibody [S71] (ab84817)
    Anti-HCN2 antibody [S71] (ab84817) at 1/1000 dilution + Rat brain membrane lysate

    Predicted band size: 95 kDa

  • Immunohistochemistry (Frozen sections) - Anti-HCN2 antibody [S71] (ab84817)
    Immunohistochemistry (Frozen sections) - Anti-HCN2 antibody [S71] (ab84817)

    Immunohistochemistry (Frozen sections) analysis of mouse brain tissue labelling HCN2 with ab84817 at 1/1000 dilution (1 hour at room temperature). Tissue fixed with 10% Formalin Solution for 12-24 hours at room temperature. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at room temperature. Counterstained with Mayer hematoxylin (purple/blue) nuclear stain at 250-500 µL for 5 minutes at room temperature.

  • Western blot - Anti-HCN2 antibody [S71] (ab84817)
    Western blot - Anti-HCN2 antibody [S71] (ab84817)
    Anti-HCN2 antibody [S71] (ab84817) at 1/1000 dilution + Mouse brain tissue lysate at 15 µg

    Secondary
    Goat anti-mouse IgG, HRP at 1/4000 dilution

    Predicted band size: 95 kDa
    Observed band size: 95 kDa



    Chemiluminescent for HRP (Moss) for 5 min in RT.

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