Anti-TRPS1 antibody (ab125197)
Key features and details
- Rabbit polyclonal to TRPS1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TRPS1 antibody
See all TRPS1 primary antibodies -
Description
Rabbit polyclonal to TRPS1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 1000-1050 of Human TRPS1 (AAG21134.1).
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Positive control
- Jurkat whole cell lysate (ab7899) can be used as a positive control in WB. Whole cell lysate from HeLa and 293T cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TRPS1 antibody (ab125197)All lanes : Anti-TRPS1 antibody (ab125197) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 142 kDa
Exposure time: 3 minutes
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Immunoprecipitation - Anti-TRPS1 antibody (ab125197)ab125197 at 1 µg/ml staining TRPS1 by WB, following immunoprecipitation of whole cell lysate from HeLa cells.
Lane 1; IP using ab125197 at 6 µg/mg lysate.
Lane 2; IP using control IgG.
1 mg of lysate was used for IP and 20% of IP was loaded. Detection utilised Chemiluminescence with a 30 second exposure. -
Western blot - Anti-TRPS1 antibody (ab125197) This image is courtesy of an anonymous AbreviewAll lanes : Anti-TRPS1 antibody (ab125197) at 1/500 dilution
Lane 1 : Ha-Ras-transformed EpH4 cells - nuclear extracts
Lanes 2-3 : EpF-2 and EpF-1 (EpH4 subclones expressing high amounts of Zeb1 and Zeb2 mRNA) - nuclear extracts
Lane 4 : EpH4 (parental) - nuclear extracts
Lane 5 : EpF-2 - nuclear extracts
Lanes 6-7 : EpF-Z1.1 and EpF-Z1.2 (EpF-1 clones transfected with siRNA to knockdown Zeb1) - nuclear extracts
Lanes 8-9 : EpF-Z2.1 and EpF-Z2.2 (EpF-1 clones transfected with siRNA to knockdown Zeb2) - nuclear extracts
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP-conjugated donkey anti-rabbit IgG polyclonal at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 142 kDa
Exposure time: 20 seconds
