Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TREM2 antibody [5F4] - BSA and Azide free (ab255881)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [5F4] to TREM2 - BSA and Azide free
  • Suitable for: ELISA, Flow Cyt
  • Reacts with: Mouse

Overview

  • Product name

    Anti-TREM2 antibody [5F4] - BSA and Azide free
    See all TREM2 primary antibodies

  • Description

    Rat monoclonal [5F4] to TREM2 - BSA and Azide free

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    ELISA
    Mouse
    Flow Cyt
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: RAW 264.7 cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab255881 is the carrier-free version of ab252876. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab255881 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Ion Exchange Chromatography

  • Clonality

    Monoclonal

  • Clone number

    5F4

  • Isotype

    IgG2a

  • Light chain type

    kappa

  • Research areas

Images

  • Flow Cytometry - Anti-TREM2 antibody [5F4] - BSA and Azide free (ab255881)
    Flow Cytometry - Anti-TREM2 antibody [5F4] - BSA and Azide free (ab255881)

    Flow cytometry analysis of Neuro-2a (Mouse neuroblastoma neuroblast, Left panel) / RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage, Right panel) labeling TREM2 using ab252876 at 1.109µg/ml (red) compared to Rat monoclonal IgG Isotype control (black) and unlabeled control cells without incubation with primary antibody and secondary antibody (blue). Secondary was Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 647, ab150163) used at a 1/2000 diluiton. Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252876).

  • ELISA - Anti-TREM2 antibody [5F4] - BSA and Azide free (ab255881)
    ELISA - Anti-TREM2 antibody [5F4] - BSA and Azide free (ab255881)

    ELISA - Anti-TREM2 antibody [5F4] (ab252876).

    Mouse TREM2 used at 1000 ng/ml.

    ab252876 used at 0-5000 ng/ml.

    Secondary is an Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rat IgG (H+L) used at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252876).

  • Anti-TREM2 antibody [5F4] - BSA and Azide free (ab255881)
    Anti-TREM2 antibody [5F4] - BSA and Azide free (ab255881)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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