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Immunology Innate Immunity Macrophage / Inflamm.

Anti-TREM2 antibody [5F4] (ab252876)

Price and availability

307 566 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-TREM2 antibody [5F4] (ab252876)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [5F4] to TREM2
  • Suitable for: Flow Cyt, ELISA
  • Reacts with: Mouse

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Overview

  • Product name

    Anti-TREM2 antibody [5F4]
    See all TREM2 primary antibodies
  • Description

    Rat monoclonal [5F4] to TREM2
  • Host species

    Rat
  • Tested Applications & Species

    Application Species
    ELISA
    Mouse
    Flow Cyt
    Mouse
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: RAW 264.7 cells.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Ion Exchange Chromatography
  • Clonality

    Monoclonal
  • Clone number

    5F4
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.

Images

  • Flow Cytometry - Anti-TREM2 antibody [5F4] (ab252876)
    Flow Cytometry - Anti-TREM2 antibody [5F4] (ab252876)

    Flow cytometry analysis of Neuro-2a (Mouse neuroblastoma neuroblast, Left panel) / RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage, Right panel) labeling TREM2 using ab252876 at 3.109µg/ml (red) compared to Rat monoclonal IgG Isotype control (black) and unlabeled control cells without incubation with primary antibody and secondary antibody (blue). Secondary was Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 647, ab150163) used at a 1/2000 diluiton. Gated on viable cells.

  • ELISA - Anti-TREM2 antibody [5F4] (ab252876)
    ELISA - Anti-TREM2 antibody [5F4] (ab252876)

    ELISA - Anti-TREM2 antibody [5F4] (ab252876).

    Mouse TREM2 used at 1000 ng/ml.

    ab252876 used at 0-5000 ng/ml.

    Secondary is an Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rat IgG (H+L) used at 1/1000 dilution.

     

  • Anti-TREM2 antibody [5F4] (ab252876)
    Anti-TREM2 antibody [5F4] (ab252876)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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