Anti-TREM2 antibody [5F4] (ab252876)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [5F4] to TREM2
- Suitable for: Flow Cyt, ELISA
- Reacts with: Mouse
Overview
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Product name
Anti-TREM2 antibody [5F4]
See all TREM2 primary antibodies -
Description
Rat monoclonal [5F4] to TREM2 -
Host species
Rat -
Tested Applications & Species
See all applications and species dataApplication Species ELISA MouseFlow Cyt Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt: RAW 264.7 cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Ion Exchange Chromatography -
Clonality
Monoclonal -
Clone number
5F4 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Flow cytometry analysis of Neuro-2a (Mouse neuroblastoma neuroblast, Left panel) / RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage, Right panel) labeling TREM2 using ab252876 at 3.109µg/ml (red) compared to Rat monoclonal IgG Isotype control (black) and unlabeled control cells without incubation with primary antibody and secondary antibody (blue). Secondary was Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 647, ab150163) used at a 1/2000 diluiton. Gated on viable cells.
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ELISA - Anti-TREM2 antibody [5F4] (ab252876).
Mouse TREM2 used at 1000 ng/ml.
ab252876 used at 0-5000 ng/ml.
Secondary is an Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rat IgG (H+L) used at 1/1000 dilution.
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