Anti-Transferrin Receptor antibody (ab216665)
Key features and details
- Rabbit polyclonal to Transferrin Receptor
- Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Transferrin Receptor antibody
See all Transferrin Receptor primary antibodies -
Description
Rabbit polyclonal to Transferrin Receptor -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide aa 620-670 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: P02786 -
Positive control
- IHC-P: Human lung carcinoma tissue. WB: Mouse placenta lysate. ICC/IF: U-138. Flow Cyt: Raji cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Transferrin Receptor antibody (ab216665)Anti-Transferrin Receptor antibody (ab216665) at 1/300 dilution + mouse placenta lysate
Developed using the ECL technique.
Predicted band size: 84 kDa
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Flow Cytometry - Anti-Transferrin Receptor antibody (ab216665)Flow cytometry analysis of Raji (Human Burkitt's lymphoma cell line) cells labeling Transferrin Receptor (green, right histogram), using ab216665 at a 1/100 dilution, and negative control cells (blue). As secondary antibody PE-conjugated was used. Secondary only (light blue) and isotope control (orange).
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Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody (ab216665)Immunofluorescence analysis of formalin-fixed U-138 (Human glioblastoma cell line) cells, labeling Transferrin Receptor using ab216665 at a 1/50 dilution; followed by secondary antibody Alexa Fluor 488-conjugated goat anti-rabbit lgG (H+L).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody (ab216665)Immunohistochemical analysis of formalin-fixed paraffin-embedded human lung carcinoma tissue, labeling Transferrin Receptor using ab216665 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
