Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Transferrin antibody [EPR18240-29] - BSA and Azide free (ab278504)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18240-29] to Transferrin - BSA and Azide free
  • Suitable for: WB, ICC, Flow Cyt (Intra)
  • Reacts with: Mouse

Overview

  • Product name

    Anti-Transferrin antibody [EPR18240-29] - BSA and Azide free
    See all Transferrin primary antibodies

  • Description

    Rabbit monoclonal [EPR18240-29] to Transferrin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC, Flow Cyt (Intra)more details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse liver, kidney, testis tissue lysate and plasma; Hepa1-6 whole cell lysate ICC: Hepa1-6 cells. Flow Cyt: Hepa1-6 (Mouse hepatoma epithelial cells)

  • General notes

    Please note: Clone [EPR18240-29] (this product) is different to that of ab277635 [EPR20149-224] for the same target.

    ab278504 is the carrier-free version of ab278498. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab278504 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Transferrin antibody [EPR18240-29] - BSA and Azide free (ab278504)
    Western blot - Anti-Transferrin antibody [EPR18240-29] - BSA and Azide free (ab278504)
    All lanes : Anti-Transferrin antibody [EPR18240-29] (ab278498) at 1/1000 dilution

    Lane 1 : Mouse liver tissue lysate
    Lane 2 : Mouse kidney tissue lysate
    Lane 3 : Mouse testis tissue lysate
    Lane 4 : Hepa1-6 (mouse hepatoma epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 77 kDa
    Observed band size: 76 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab278498, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: Lane 1-3: 3.25 seconds; Lane 4: 70 seconds

  • Western blot - Anti-Transferrin antibody [EPR18240-29] - BSA and Azide free (ab278504)
    Western blot - Anti-Transferrin antibody [EPR18240-29] - BSA and Azide free (ab278504)
    Anti-Transferrin antibody [EPR18240-29] (ab278498) at 1/1000 dilution + Mouse plasma at 20 µl

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 77 kDa
    Observed band size: 76 kDa why is the actual band size different from the predicted?



    This data was developed using ab278498, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 10 seconds.

  • Immunocytochemistry - Anti-Transferrin antibody [EPR18240-29] - BSA and Azide free (ab278504)
    Immunocytochemistry - Anti-Transferrin antibody [EPR18240-29] - BSA and Azide free (ab278504)

    This data was developed using ab278498, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Hepa1-6 cells labelling Transferrin with ab278498 at 1/100 (5.52 μg/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in Hepa1-6 cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-Transferrin antibody [EPR18240-29] - BSA and Azide free (ab278504)
    Flow Cytometry (Intracellular) - Anti-Transferrin antibody [EPR18240-29] - BSA and Azide free (ab278504)

    This data was developed using ab278498, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Hepa1-6 (Mouse hepatoma epithelial cell) cells labelling Transferrin with ab278498 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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