Anti-TRAM1/TRAM antibody - C-terminal (ab190982)
Key features and details
- Rabbit polyclonal to TRAM1/TRAM - C-terminal
- Suitable for: WB, IHC-P, ICC, IHC-Fr
- Reacts with: Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-TRAM1/TRAM antibody - C-terminal
See all TRAM1/TRAM primary antibodies -
Description
Rabbit polyclonal to TRAM1/TRAM - C-terminal -
Host species
Rabbit -
Specificity
No cross reactivity with other proteins. -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanIHC-Fr RatIHC-P RatHumanWB Rat
-
Immunogen
Synthetic peptide corresponding to Human TRAM1/TRAM aa 300 to the C-terminus (C terminal).
Database link: Q15629 -
Positive control
- Rat brain, rat kidney, 293T, Raji and Jurkat lysates; Human lung cancer and rat kidney tissues; HeLa cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAM1/TRAM antibody - C-terminal (ab190982)
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling TRAM1 with ab190982 at 1 µg/ml.
-
Western blot - Anti-TRAM1/TRAM antibody - C-terminal (ab190982)All lanes : Anti-TRAM1/TRAM antibody - C-terminal (ab190982)
Lane 1 : Rat brain tissue lysate
Lane 2 : Rat kidney tissue lysate
Lane 3 : 293T cell lysate
Lane 4 : RAJI cell lysate
Lane 5 : Jurkat cell lysate
Lane 6 : HeLa cell lysate
Predicted band size: 43 kDa
-
Immunohistochemistry (Frozen sections) - Anti-TRAM1/TRAM antibody - C-terminal (ab190982)TRAM1 was detected in frozen section of rat brain tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab190982 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAM1/TRAM antibody - C-terminal (ab190982)Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling TRAM1 with ab190982 at 1 µg/ml.
-
Immunocytochemistry - Anti-TRAM1/TRAM antibody - C-terminal (ab190982)Immunocytochemical analysis of HeLa cells labeling TRAM1 with ab190982 at 1 µg/ml.
-
Immunohistochemistry (Frozen sections) - Anti-TRAM1/TRAM antibody - C-terminal (ab190982)Immunohistochemical analysis of frozen sections of rat instestine tissues staining TRAM1 with ab190982 at 1µg/ml. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. Antibody incubation was carried out overnight. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
