Anti-TPR antibody (ab264153)
Key features and details
- Rabbit polyclonal to TPR
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TPR antibody
See all TPR primary antibodies -
Description
Rabbit polyclonal to TPR -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human TPR aa 2300-2349 (C terminal). The exact sequence is proprietary. NP_003283.1
Database link: P12270 -
Positive control
- IHC-P: Human testis tissue. WB: HeLa and HEK-293T whole cell lysate. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TPR antibody (ab264153)All lanes : Anti-TPR antibody (ab264153) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T whole cell lysate at 50 µg
Predicted band size: 267 kDa
Exposure time: 30 seconds4-20% SDS-PAGE
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Immunoprecipitation - Anti-TPR antibody (ab264153)TPR was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264153 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264153 at 1 µg/ml.
Lane 1: ab264153 IP in HeLa whole cell lysate.
Lane 2: Control IgG.Exposure time: 1 sec.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPR antibody (ab264153)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human testis tissue labeling TPR with ab264153 at 1/100 dilution. Detection: DAB.
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
