Anti-TOX antibody [NAN448B] (ab237009)
![Anti-TOX antibody [NAN448B] (ab237009)](https://www.abcam.com/ps/products/237/ab237009/Images/ab237009-356511-anti-tox1-antibody-nan448b-western-blot-molt-4-human.JPG "Anti-TOX antibody [NAN448B] (ab237009)")
Key features and details
- Rat monoclonal [NAN448B] to TOX
- Suitable for: WB, IHC-P, IHC-Fr
- Knockout validated
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-TOX antibody [NAN448B]
See all TOX primary antibodies -
Description
Rat monoclonal [NAN448B] to TOX -
Host species
Rat -
Tested Applications & Species
See all applications and species dataApplication Species IHC-Fr HumanIHC-P MouseHumanWB Human
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Immunogen
Recombinant fragment corresponding to Human TOX aa 1-250.
Database link: O94900 -
Positive control
- WB: MOLT4 cell lysate IHC-P: Human tonsil, mycosis fungoides, NLPHL, fetal liver, thymus, kidney and testicle tissues. Mouse brain, retina, pancreas, spleen and thymus.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
NAN448B -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-TOX antibody [NAN448B] (ab237009)All lanes : Anti-TOX antibody [NAN448B] (ab237009) at 1/200 dilution
Lane 1 : MOLT 4 cell line
Lane 2 : MOLT 4 KO cell line
Lysates/proteins at 100 µg per lane.
Predicted band size: 58 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [NAN448B] (ab237009)](https://www.abcam.com/ps/products/237/ab237009/Images/ab237009-356568-anti-tox1-antibody-nan448b-immunocytochemistry-immunofluorecence-human-tonsil.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [NAN448B] (ab237009)Immunofluorescent analysis of paraffin-embedded human tonsil tissue labelling TOX with ab237009 at 1/50 dilution (Red). DAPI is stained in blue and Ki67 is stained in green.
Antigen retrieval: 20 minutes ER2 (Tris-EDTA)
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [NAN448B] (ab237009)](https://www.abcam.com/ps/products/237/ab237009/Images/ab237009-315076-anti-tox1-antibody-nan448b-immunohistochemistry-tonsil-human.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [NAN448B] (ab237009)Paraffin-embedded human tonsil tissue stained for TOX using ab237009 at 1/200 dilution in immunohistochemical analysis.
Antigen retrieval: 20 minutes ER2 (Tris-EDTA)
Incubated with primary antibody for 30 minutes.
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![Immunohistochemistry (Frozen sections) - Anti-TOX antibody [NAN448B] (ab237009)](https://www.abcam.com/ps/products/237/ab237009/Images/ab237009-315074-anti-tox1-antibody-nan448b-immunocytochemistry-hek293-human.JPG)
Immunohistochemistry (Frozen sections) - Anti-TOX antibody [NAN448B] (ab237009)To confirm that NAN448B mAb recognized human TOX protein and showed no cross-reactivity to other TOX family members, immunohistochemistry on frozen cytospins preparations of TOX1 ,TOX2 ,TOX3 and TOX4 expressed in HEK293T cells was performed. Anti-myc and anti-GFP antibodies were used as positive controls.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [NAN448B] (ab237009)](https://www.abcam.com/ps/products/237/ab237009/Images/ab237009-356564-anti-tox1-antibody-nan448b-immunohistochemistry-human-tonsil.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [NAN448B] (ab237009)Paraffin-embedded human tonsil tissue stained for TOX using ab237009 at 1/200 dilution in immunohistochemical analysis.
Antigen retrieval: 20 minutes ER2 (Tris-EDTA)
Incubated with primary antibody for 30 minutes.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [NAN448B] (ab237009)](https://www.abcam.com/ps/products/237/ab237009/Images/ab237009-315080-anti-tox1-antibody-nan448b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [NAN448B] (ab237009)Paraffin-embedded human testicle tissue stained for TOX using ab237009 at 1/300 dilution in immunohistochemical analysis.
Antigen retrieval: ER2 for 20 minutes.
Incubated with primary antibody for 30 minutes.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [NAN448B] (ab237009)](https://www.abcam.com/ps/products/237/ab237009/Images/ab237009-356567-anti-tox1-antibody-nan448b-immunocytochemistry-immunofluorecence-tonsil-human.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [NAN448B] (ab237009)Immunofluorescent analysis of paraffin-embedded human tonsil tissue labelling TOX with ab237009 at 1/50 dilution (Red). DAPI is stained in blue and Ki67 is stained in green.
Antigen retrieval: 20 minutes ER2 (Tris-EDTA)
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [NAN448B] (ab237009)](https://www.abcam.com/ps/products/237/ab237009/Images/ab237009-356566-anti-tox1-antibody-nan448b-immunohistochemistry-nodular-lymphocyte-predominant-hodgkin-human.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [NAN448B] (ab237009)Paraffin-embedded Nodular Lymphocyte predominant Hodgkin Lymphoma (NLPHL) tissue stained for TOX using ab237009 at 1/200 dilution in immunohistochemical analysis. PD1 is stained in red.
Antigen retrieval: 20 minutes ER2 (Tris-EDTA).
Incubated with primary antibody for 30 minutes.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [NAN448B] (ab237009)](https://www.abcam.com/ps/products/237/ab237009/Images/ab237009-356513-anti-tox1-antibody-nan448b-immunohistochemistry-brain-retina.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [NAN448B] (ab237009)Paraffin-embedded mouse retina tissue stained for TOX using ab237009 at 1/100 dilution in immunohistochemical analysis.
Antigen retrieval: pH high
Incubated with primary antibody for 20 minutes.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [NAN448B] (ab237009)](https://www.abcam.com/ps/products/237/ab237009/Images/ab237009-315079-anti-tox1-antibody-nan448b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [NAN448B] (ab237009)Paraffin-embedded human kidney tissue stained for TOX using ab237009 at 1/300 dilution in immunohistochemical analysis.
Antigen retrieval: ER2 for 20 minutes.
Incubated with primary antibody for 30 minutes.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [NAN448B] (ab237009)](https://www.abcam.com/ps/products/237/ab237009/Images/ab237009-356565-anti-tox1-antibody-nan448b-immunohistochemistry-mycosis-fungoide.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [NAN448B] (ab237009)Paraffin-embedded mycosis fungoide tissue stained for TOX using ab237009 at 1/200 dilution in immunohistochemical analysis. CD8 is stained in red.
Antigen retrieval: 20 minutes ER2 (Tris-EDTA).
Incubated with primary antibody for 30 minutes.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [NAN448B] (ab237009)](https://www.abcam.com/ps/products/237/ab237009/Images/ab237009-356512-anti-tox1-antibody-nan448b-immunohistochemistry-brain-mouse.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [NAN448B] (ab237009)Paraffin-embedded mouse brain tissue stained for TOX using ab237009 at 1/100 dilution in immunohistochemical analysis.
Antigen retrieval: pH high
Incubated with primary antibody for 20 minutes.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [NAN448B] (ab237009)](https://www.abcam.com/ps/products/237/ab237009/Images/ab237009-356514-anti-tox1-antibody-nan448b-immunohistochemistry-pancreas-retina.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [NAN448B] (ab237009)Paraffin-embedded mouse pancreas tissue stained for TOX using ab237009 at 1/100 dilution in immunohistochemical analysis.
Antigen retrieval: pH high
Incubated with primary antibody for 20 minutes.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [NAN448B] (ab237009)](https://www.abcam.com/ps/products/237/ab237009/Images/ab237009-356515-anti-tox1-antibody-nan448b-immunohistochemistry-thymus-human.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [NAN448B] (ab237009)Paraffin-embedded mouse thymus tissue stained for TOX using ab237009 at 1/100 dilution in immunohistochemical analysis.
Antigen retrieval: pH high
Incubated with primary antibody for 20 minutes.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [NAN448B] (ab237009)](https://www.abcam.com/ps/products/237/ab237009/Images/ab237009-332980-anti-tox1-antibody-nan448b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [NAN448B] (ab237009)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse spleen staining TOX1 with purified ab237009 at 1:100 for 20 mins. Antigen retrival was performed using a high pH.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [NAN448B] (ab237009)](https://www.abcam.com/ps/products/237/ab237009/Images/ab237009-315077-anti-tox1-antibody-nan448b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOX antibody [NAN448B] (ab237009)Paraffin-embedded human fetal liver tissue stained for TOX using ab237009 at 1/300 dilution in immunohistochemical analysis.
Antigen retrieval: ER2 for 20 minutes.
Incubated with primary antibody for 30 minutes.
