Anti-TOM1 antibody [EPR11988(B)] - BSA and Azide free (ab249549)
![Anti-TOM1 antibody [EPR11988(B)] - BSA and Azide free (ab249549)](https://www.abcam.com/ps/products/249/ab249549/Images/ab249549-365881-anti-tom1-antibody-epr11988b-western-blot-knockout-hap1-tom1-hepg2-hela-human-lysates.jpg "Anti-TOM1 antibody [EPR11988(B)] - BSA and Azide free (ab249549)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11988(B)] to TOM1 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-TOM1 antibody [EPR11988(B)] - BSA and Azide free
See all TOM1 primary antibodies -
Description
Rabbit monoclonal [EPR11988(B)] to TOM1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab249549 is the carrier-free version of ab170866. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab249549 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR11988(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TOM1 antibody [EPR11988(B)] - BSA and Azide free (ab249549)All lanes : Anti-TOM1 antibody [EPR11988(B)] (ab170866) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : TOM1 knockout HAP1 whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lane 4 : HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 54 kDaThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170866).
Lanes 1 - 4: Merged signal (red and green). Green - ab170866 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab170866 was shown to recognize in wild-type HAP1 cells as signal was lost at the expected MW in TOM1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and TOM1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. Ab170866 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Anti-TOM1 antibody [EPR11988(B)] - BSA and Azide free (ab249549)](https://www.abcam.com/ps/products/249/ab249549/Images/ab249549-2-benefits-of-recombinant-antibodies.png)
Anti-TOM1 antibody [EPR11988(B)] - BSA and Azide free (ab249549)