Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] - BSA and Azide free (ab248657)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2476] to PDGFR beta (phospho Y1021) - BSA and Azide free
  • Suitable for: Dot blot, WB, Flow Cyt
  • Reacts with: Mouse

Overview

  • Product name

    Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] - BSA and Azide free
    See all PDGFR beta primary antibodies

  • Description

    Rabbit monoclonal [EPR2476] to PDGFR beta (phospho Y1021) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    Only detects PDGFR beta phosphorylated at tyrosine 1021.

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248657 is the carrier-free version of ab134048 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab248657 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] - BSA and Azide free (ab248657)
    Western blot - Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] - BSA and Azide free (ab248657)
    All lanes : Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] (ab134048) at 1/5000 dilution (Purified)

    Lane 1 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 2 : NIH/3T3 treated with 1mM pervanadate for 30 minutes whole cell lysate
    Lane 3 : NIH/3T3 treated with 1mM pervanadate for 30 minutes whole cell lysate. Then the membrane was incubated with alkaline phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 190 kDa



    This data was developed using ab134048, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

     

  • Dot Blot - Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] - BSA and Azide free (ab248657)
    Dot Blot - Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] - BSA and Azide free (ab248657)
    This data was developed using ab134048, the same antibody clone in a different buffer formulation.Dot blot analysis of PDGFR beta (phospho Y1021) phospho peptide (Lane 1) and PDGFR beta non-phospho peptide (Lane 2) labeling PDGFR beta (phospho Y1021) phospho peptide with ab134048 at a dilution of 1/1000 dilution (0.54ug/ml). A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000 dilution. Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST .
  • Western blot - Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] - BSA and Azide free (ab248657)
    Western blot - Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] - BSA and Azide free (ab248657)
    All lanes : Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] (ab134048) at 1/50000 dilution

    Lane 1 : NIH/3T3 untreated
    Lane 2 : NIH/3T3 treated with Pervanadate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 190 kDa



    This data was developed using ab134048, the same antibody clone in a different buffer formulation.

     

  • Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] - BSA and Azide free (ab248657)
    Anti-PDGFR beta (phospho Y1021) antibody [EPR2476] - BSA and Azide free (ab248657)

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