Anti-TNF alpha antibody (ab183896)
Key features and details
- Rabbit Polyclonal to TNF alpha
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TNF alpha antibody
See all TNF alpha primary antibodies -
Description
Rabbit Polyclonal to TNF alpha -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human TNF alpha aa 1-233. The exact sequence is proprietary.
Database link: P01375 -
Positive control
- Human gastric carcinoma and HeLa xenograft tissues. Jurkat cells. Raji, 293T and TNF-transfected 293T whole cell lysates/extracts
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TNF alpha antibody (ab183896)Anti-TNF alpha antibody (ab183896) at 1/1000 dilution + Raji whole cell lysate/extract at 30 µg
Predicted band size: 26 kDa
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Western blot - Anti-TNF alpha antibody (ab183896)All lanes : Anti-TNF alpha antibody (ab183896) at 1/5000 dilution
Lane 1 : 293T whole cell lysate/extract
Lane 2 : TNF alpha-transfected 293T whole cell lysate/extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 26 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF alpha antibody (ab183896)Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma, labeling TNF alpha using ab183896 at a 1/500 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNF alpha antibody (ab183896)Immunohistochemical analysis of paraffin-embedded HeLa xenograft, labeling TNF alpha using ab183896 at a 1/500 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody (ab183896)Immunofluorescence analysis of Jurkat cells fixed in 4% paraformaldehyde at RT for 15 minutes, labeling TNF alpha using ab183896 at a 1/500 dilution (green). Blue: Hoechst 33342 staining.
