Anti-TMEM120B antibody (ab121413)
Key features and details
- Rabbit polyclonal to TMEM120B
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TMEM120B antibody -
Description
Rabbit polyclonal to TMEM120B -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen: THRIYKQKLE ELAALQTLCS SSISKQKKHL KDLKLTLQRC KRHASREEAE LVQQMAANIK ERQDVFFDME AYLPKKNGLY LNLVLGNVNV TLLSNQAKFA YKD, corresponding to amino acids 25-127 of Human TMEM120B (A0PK00).
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Positive control
- Human stomach tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TMEM120B antibody (ab121413)Lane 1 : Anti-TMEM120B antibody (ab121413) at 1/100 dilution
Lane 2 : Anti-TMEM120B antibody (ab121413)
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : Over-expression lysate (co-expressed with a C-terminal myc-DDK tag (~3.1kDa) in HEK293T cells.
Predicted band size: 40 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-TMEM120B antibody (ab121413)Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleoli and cytoplasm. Recommended concentration of ab121413 1-4 µg/ml. Cells treated with PFA/Triton X-100. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM120B antibody (ab121413)Immunohistochemical staining of Human stomach tissue shows strong cytoplasmic positivity in glandular cells, using 1/20 ab121413.