Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TM9SF2 antibody [EPR24416-17] - BSA and Azide free (ab281838)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24416-17] to TM9SF2 - BSA and Azide free
  • Suitable for: WB, IHC-P, IHC-Fr, Flow Cyt (Intra)

Overview

  • Product name

    Anti-TM9SF2 antibody [EPR24416-17] - BSA and Azide free
    See all TM9SF2 primary antibodies

  • Description

    Rabbit monoclonal [EPR24416-17] to TM9SF2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IHC-Fr, Flow Cyt (Intra)more details
    Unsuitable for: ICC or IP

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HT-29, PANC-1, U-87 MG, C6, PC-12, HEK-293T transfected with human TM9SF2 expression vector containing a myc-His-tag® cell lysates, Human kidney, Mouse kidney, colon, Rat colon tissue lysates. IHC-P: Human kidney, Mouse pancreas, Rat pancreas tissues. IHC-Fr: Mouse kidney, Rat kidney tissues. Flow Cyt: U-87 MG cells.

  • General notes

    ab281838 is the carrier-free version of ab271123. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab281838 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR24416-17

  • Isotype

    IgG

Images

  • Western blot - Anti-TM9SF2 antibody [EPR24416-17] - BSA and Azide free (ab281838)
    Western blot - Anti-TM9SF2 antibody [EPR24416-17] - BSA and Azide free (ab281838)
    All lanes : Anti-TM9SF2 antibody [EPR24416-17] (ab271123) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma), whole cell lysate
    Lane 2 : HT-29 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
    Lane 3 : PANC-1 (human pancreatic epithelioid carcinoma epithelial cell), whole cell lysate
    Lane 4 : U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate
    Lane 5 : Human kidney tissue lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/2000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated; Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG)

    Predicted band size: 76 kDa
    Observed band size: 55 kDa why is the actual band size different from the predicted?



    This data was developed using 271123, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 3 minutes

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TM9SF2 antibody [EPR24416-17] - BSA and Azide free (ab281838)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TM9SF2 antibody [EPR24416-17] - BSA and Azide free (ab281838)

    This data was developed using 271123, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling TM9SF2 with ab271123 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human kidney. The section was incubated with ab271123 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Flow Cytometry - Anti-TM9SF2 antibody [EPR24416-17] - BSA and Azide free (ab281838)
    Flow Cytometry - Anti-TM9SF2 antibody [EPR24416-17] - BSA and Azide free (ab281838)

    This data was developed using 271123, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized U-87 MG (Human glioblastoma-astrocytoma epithelial cell) cells labelling  with ab271123 at 1/50 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.

  • Western blot - Anti-TM9SF2 antibody [EPR24416-17] - BSA and Azide free (ab281838)
    Western blot - Anti-TM9SF2 antibody [EPR24416-17] - BSA and Azide free (ab281838)
    All lanes : Anti-TM9SF2 antibody [EPR24416-17] (ab271123) at 1/1000 dilution

    Lane 1 : C6 (rat glial tumor glial cell), whole cell lysate
    Lane 2 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
    Lane 3 : Mouse kidney tissue lysate
    Lane 4 : Mouse colon tissue lysate
    Lane 5 : Rat colon tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 76 kDa
    Observed band size: 55 kDa why is the actual band size different from the predicted?



    This data was developed using 271123, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Samples are non-boiled as boiling may cause protein aggregates.

    Exposure time 3 minutes

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TM9SF2 antibody [EPR24416-17] - BSA and Azide free (ab281838)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TM9SF2 antibody [EPR24416-17] - BSA and Azide free (ab281838)

    This data was developed using 271123, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labelling TM9SF2 with ab271123 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse pancreas. The section was incubated with ab271123 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Counterstained with Hematoxylin. 

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Frozen sections) - Anti-TM9SF2 antibody [EPR24416-17] - BSA and Azide free (ab281838)
    Immunohistochemistry (Frozen sections) - Anti-TM9SF2 antibody [EPR24416-17] - BSA and Azide free (ab281838)

    This data was developed using 271123, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney tissue labelling TM9SF2 with ab271123 at 1100 (5.93 μg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Cytoplasmic staining on mouse kidney is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Western blot - Anti-TM9SF2 antibody [EPR24416-17] - BSA and Azide free (ab281838)
    Western blot - Anti-TM9SF2 antibody [EPR24416-17] - BSA and Azide free (ab281838)
    All lanes : Anti-TM9SF2 antibody [EPR24416-17] (ab271123) at 1/1000 dilution

    Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector, containing a myc-His-tag®, whole cell lysate
    Lane 2 : HEK-293T transfected with human TM9SF2 expression vector containing a myc-His-tag®, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 76 kDa
    Observed band size: 55 kDa why is the actual band size different from the predicted?



    This data was developed using 271123, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Band higher than 250 kDa should be TM9SF2 aggregates.

    Exposure time: 10 seconds

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TM9SF2 antibody [EPR24416-17] - BSA and Azide free (ab281838)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TM9SF2 antibody [EPR24416-17] - BSA and Azide free (ab281838)

    This data was developed using 271123, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat pancreas tissue labelling TM9SF2 with ab271123 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat pancreas. The section was incubated with ab271123for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Counterstained with Hematoxylin. 

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Frozen sections) - Anti-TM9SF2 antibody [EPR24416-17] - BSA and Azide free (ab281838)
    Immunohistochemistry (Frozen sections) - Anti-TM9SF2 antibody [EPR24416-17] - BSA and Azide free (ab281838)

    This data was developed using 271123, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat kidney tissue labelling TM9SF2 with ab271123 at 1100 (5.93 μg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Cytoplasmic staining on rat kidney is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Anti-TM9SF2 antibody [EPR24416-17] - BSA and Azide free (ab281838)
    Anti-TM9SF2 antibody [EPR24416-17] - BSA and Azide free (ab281838)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Clear all

Recently viewed products

© 2021 Astana Biomed Group LLP. All rights reserved.