Anti-Tissue Factor antibody [EPR22548-240] (ab252918)
![Anti-Tissue Factor antibody [EPR22548-240] (ab252918)](https://www.abcam.com/ps/products/252/ab252918/Images/ab252918-2-252918IHC2.jpg "Anti-Tissue Factor antibody [EPR22548-240] (ab252918)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22548-240] to Tissue Factor
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Tissue Factor antibody [EPR22548-240]
See all Tissue Factor primary antibodies -
Description
Rabbit monoclonal [EPR22548-240] to Tissue Factor -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HAP1, A431, MDA-MB-231 and BxPC-3 whole cell lysates; human brain cell, heart cell and fetal kidney cell lysates. IHC-P: Human kidney and cervix carcinoma tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22548-240 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Factor antibody [EPR22548-240] (ab252918)
Immunohistochemical analysis of paraffin-embedded human cervix carcinoma tissue labeling Tissue Factor with ab252918 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous and weak cytoplasmic staining on tumor cells of human cervix carcinoma (PMID: 26383146) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 minutes.
The section was incubated with ab252918 for 30 minutess at RT.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Factor antibody [EPR22548-240] (ab252918)](https://www.abcam.com/ps/products/252/ab252918/Images/ab252918-1-252918IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Factor antibody [EPR22548-240] (ab252918)Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Tissue Factor with ab252918 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human renal glomerulus (PMID: 7684196) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 minutes.
The section was incubated with ab252918 for 30 minutes at RT.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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![Western blot - Anti-Tissue Factor antibody [EPR22548-240] (ab252918)](https://www.abcam.com/ps/products/252/ab252918/Images/ab252918-3-252918WB1.jpg)
Western blot - Anti-Tissue Factor antibody [EPR22548-240] (ab252918)All lanes : Anti-Tissue Factor antibody [EPR22548-240] (ab252918) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate at 60 µg
Lane 2 : Tissue Factor knockout HAP1 whole cell lysate at 60 µg
Lane 3 : A431 (human epidermoid carcinoma cell line) whole cell lysate at 20 µg
Lane 4 : MDA-MB-231 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 5 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 6 : BxPC-3 (human pancreas adenocarcinoma epithelial cell line) whole cell lysate at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 33 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Tissue Factor antibody [EPR22548-240] (ab252918)](https://www.abcam.com/ps/products/252/ab252918/Images/ab252918-4-252918WB2.jpg)
Western blot - Anti-Tissue Factor antibody [EPR22548-240] (ab252918)All lanes : Anti-Tissue Factor antibody [EPR22548-240] (ab252918) at 1/1000 dilution
Lane 1 : Human brain cell lysate
Lane 2 : Human heart cell lysate
Lane 3 : Human fetal kidney cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 33 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted?
Exposure time: 8 secondsBlocking and dilution buffer: 5% NFDM/TBST.
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![Anti-Tissue Factor antibody [EPR22548-240] (ab252918)](https://www.abcam.com/ps/products/252/ab252918/Images/ab252918-5-benefits-of-recombinant-antibodies.png)
Anti-Tissue Factor antibody [EPR22548-240] (ab252918)