Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Tissue Factor antibody [EPR22548-240] (ab252918)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22548-240] to Tissue Factor
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Tissue Factor antibody [EPR22548-240]
    See all Tissue Factor primary antibodies

  • Description

    Rabbit monoclonal [EPR22548-240] to Tissue Factor

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HAP1, A431, MDA-MB-231 and BxPC-3 whole cell lysates; human brain cell, heart cell and fetal kidney cell lysates. IHC-P: Human kidney and cervix carcinoma tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Factor antibody [EPR22548-240] (ab252918)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Factor antibody [EPR22548-240] (ab252918)

    Immunohistochemical analysis of paraffin-embedded human cervix carcinoma tissue labeling Tissue Factor with ab252918 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous and weak cytoplasmic staining on tumor cells of human cervix carcinoma (PMID: 26383146) is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 minutes.

    The section was incubated with ab252918 for 30 minutess at RT.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Factor antibody [EPR22548-240] (ab252918)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Factor antibody [EPR22548-240] (ab252918)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Tissue Factor with ab252918 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human renal glomerulus (PMID: 7684196) is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 minutes.

    The section was incubated with ab252918 for 30 minutes at RT.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Western blot - Anti-Tissue Factor antibody [EPR22548-240] (ab252918)
    Western blot - Anti-Tissue Factor antibody [EPR22548-240] (ab252918)
    All lanes : Anti-Tissue Factor antibody [EPR22548-240] (ab252918) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate at 60 µg
    Lane 2 : Tissue Factor knockout HAP1 whole cell lysate at 60 µg
    Lane 3 : A431 (human epidermoid carcinoma cell line) whole cell lysate at 20 µg
    Lane 4 : MDA-MB-231 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg
    Lane 5 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg
    Lane 6 : BxPC-3 (human pancreas adenocarcinoma epithelial cell line) whole cell lysate at 20 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 33 kDa
    Observed band size: 47 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Tissue Factor antibody [EPR22548-240] (ab252918)
    Western blot - Anti-Tissue Factor antibody [EPR22548-240] (ab252918)
    All lanes : Anti-Tissue Factor antibody [EPR22548-240] (ab252918) at 1/1000 dilution

    Lane 1 : Human brain cell lysate
    Lane 2 : Human heart cell lysate
    Lane 3 : Human fetal kidney cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 33 kDa
    Observed band size: 47 kDa why is the actual band size different from the predicted?


    Exposure time: 8 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-Tissue Factor antibody [EPR22548-240] (ab252918)
    Anti-Tissue Factor antibody [EPR22548-240] (ab252918)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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